<?xml version="1.0" encoding="UTF-8"?>

<!--XBRL Document Created with the Workiva Platform-->
<!--Copyright 2024 Workiva-->
<!--r:49e8c184-bfae-4e40-8964-df64067936eb,g:2a0d9318-df54-4d64-82e1-c75e07354b2c-->
<link:linkbase xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:xbrldt="http://xbrl.org/2005/xbrldt" xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd">
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/all" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#all"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#hypercube-dimension"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-default"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-domain"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/domain-member" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#domain-member"/>
  <link:roleRef roleURI="http://www.centrusenergy.com/role/Cover" xlink:type="simple" xlink:href="leu-20240630.xsd#Cover"/>
  <link:definitionLink xlink:role="http://www.centrusenergy.com/role/Cover" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentInformationLineItems_8abdae0b-1524-45e4-89f5-5cce0fe00d2b" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_DocumentInformationLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentInformationTable_926a663b-c335-45c7-9773-d7a6c0885f53" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_DocumentInformationTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_dei_DocumentInformationLineItems_8abdae0b-1524-45e4-89f5-5cce0fe00d2b" xlink:to="loc_dei_DocumentInformationTable_926a663b-c335-45c7-9773-d7a6c0885f53" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementClassOfStockAxis_61f6199c-f145-4ab2-8d8e-002f92a2d785" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementClassOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_dei_DocumentInformationTable_926a663b-c335-45c7-9773-d7a6c0885f53" xlink:to="loc_us-gaap_StatementClassOfStockAxis_61f6199c-f145-4ab2-8d8e-002f92a2d785" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_61f6199c-f145-4ab2-8d8e-002f92a2d785_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementClassOfStockAxis_61f6199c-f145-4ab2-8d8e-002f92a2d785" xlink:to="loc_us-gaap_ClassOfStockDomain_61f6199c-f145-4ab2-8d8e-002f92a2d785_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_66fc3046-c611-4004-97cb-c6a92b093d5d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementClassOfStockAxis_61f6199c-f145-4ab2-8d8e-002f92a2d785" xlink:to="loc_us-gaap_ClassOfStockDomain_66fc3046-c611-4004-97cb-c6a92b093d5d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonClassAMember_ac6078c6-86c9-40bb-a5f0-75094a598137" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommonClassAMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_66fc3046-c611-4004-97cb-c6a92b093d5d" xlink:to="loc_us-gaap_CommonClassAMember_ac6078c6-86c9-40bb-a5f0-75094a598137" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonClassBMember_5c7eb43d-0399-4277-a8f6-c8e6dd57c851" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommonClassBMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_66fc3046-c611-4004-97cb-c6a92b093d5d" xlink:to="loc_us-gaap_CommonClassBMember_5c7eb43d-0399-4277-a8f6-c8e6dd57c851" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentType_cfab9130-21e4-4fa9-82c8-22b02bfc854c" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_DocumentType"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_8abdae0b-1524-45e4-89f5-5cce0fe00d2b" xlink:to="loc_dei_DocumentType_cfab9130-21e4-4fa9-82c8-22b02bfc854c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentQuarterlyReport_6277d924-1837-467c-a0fd-346e993c5553" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_DocumentQuarterlyReport"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_8abdae0b-1524-45e4-89f5-5cce0fe00d2b" xlink:to="loc_dei_DocumentQuarterlyReport_6277d924-1837-467c-a0fd-346e993c5553" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentPeriodEndDate_d5b752d4-13a8-498b-93d6-fdd403e26816" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_DocumentPeriodEndDate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_8abdae0b-1524-45e4-89f5-5cce0fe00d2b" xlink:to="loc_dei_DocumentPeriodEndDate_d5b752d4-13a8-498b-93d6-fdd403e26816" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentTransitionReport_c8422f2d-bc8f-462b-a8dd-c5525479b595" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_DocumentTransitionReport"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_8abdae0b-1524-45e4-89f5-5cce0fe00d2b" xlink:to="loc_dei_DocumentTransitionReport_c8422f2d-bc8f-462b-a8dd-c5525479b595" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityFileNumber_3e8e1b87-6952-44db-a2f2-2871c7aabf68" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityFileNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_8abdae0b-1524-45e4-89f5-5cce0fe00d2b" xlink:to="loc_dei_EntityFileNumber_3e8e1b87-6952-44db-a2f2-2871c7aabf68" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityRegistrantName_6d6794de-29da-48af-ae8c-4f69f149af0d" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityRegistrantName"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_8abdae0b-1524-45e4-89f5-5cce0fe00d2b" xlink:to="loc_dei_EntityRegistrantName_6d6794de-29da-48af-ae8c-4f69f149af0d" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityIncorporationStateCountryCode_05009778-6cf4-4266-a50b-6367d586196d" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityIncorporationStateCountryCode"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_8abdae0b-1524-45e4-89f5-5cce0fe00d2b" xlink:to="loc_dei_EntityIncorporationStateCountryCode_05009778-6cf4-4266-a50b-6367d586196d" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityTaxIdentificationNumber_e14c5b65-4f4c-46a2-820c-9f35e2826ea8" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityTaxIdentificationNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_8abdae0b-1524-45e4-89f5-5cce0fe00d2b" xlink:to="loc_dei_EntityTaxIdentificationNumber_e14c5b65-4f4c-46a2-820c-9f35e2826ea8" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressAddressLine1_3642b0cb-0932-4e16-b793-b0612308a208" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityAddressAddressLine1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_8abdae0b-1524-45e4-89f5-5cce0fe00d2b" xlink:to="loc_dei_EntityAddressAddressLine1_3642b0cb-0932-4e16-b793-b0612308a208" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressAddressLine2_1af97308-3224-48dd-820e-98620a61e13d" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityAddressAddressLine2"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_8abdae0b-1524-45e4-89f5-5cce0fe00d2b" xlink:to="loc_dei_EntityAddressAddressLine2_1af97308-3224-48dd-820e-98620a61e13d" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressCityOrTown_db46e1dd-9c0a-4980-9518-57add1422c61" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityAddressCityOrTown"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_8abdae0b-1524-45e4-89f5-5cce0fe00d2b" xlink:to="loc_dei_EntityAddressCityOrTown_db46e1dd-9c0a-4980-9518-57add1422c61" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressStateOrProvince_39e10d6a-5322-4181-8d69-2149e7a38fdc" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityAddressStateOrProvince"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_8abdae0b-1524-45e4-89f5-5cce0fe00d2b" xlink:to="loc_dei_EntityAddressStateOrProvince_39e10d6a-5322-4181-8d69-2149e7a38fdc" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressPostalZipCode_37acbea9-01cf-420d-bcdc-bbbb87f26354" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityAddressPostalZipCode"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_8abdae0b-1524-45e4-89f5-5cce0fe00d2b" xlink:to="loc_dei_EntityAddressPostalZipCode_37acbea9-01cf-420d-bcdc-bbbb87f26354" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_CityAreaCode_c35ec360-0eaf-4173-97bd-4f0d93df5bd4" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_CityAreaCode"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_8abdae0b-1524-45e4-89f5-5cce0fe00d2b" xlink:to="loc_dei_CityAreaCode_c35ec360-0eaf-4173-97bd-4f0d93df5bd4" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LocalPhoneNumber_80543c72-247e-4669-ab35-2768ebec2175" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_LocalPhoneNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_8abdae0b-1524-45e4-89f5-5cce0fe00d2b" xlink:to="loc_dei_LocalPhoneNumber_80543c72-247e-4669-ab35-2768ebec2175" xlink:type="arc" order="14"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_Security12bTitle_d3aa2f3d-6dae-42fe-8217-4740be9d5662" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_Security12bTitle"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_8abdae0b-1524-45e4-89f5-5cce0fe00d2b" xlink:to="loc_dei_Security12bTitle_d3aa2f3d-6dae-42fe-8217-4740be9d5662" xlink:type="arc" order="15"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_TradingSymbol_058f81c7-d191-48eb-8433-06aa27d56ee0" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_TradingSymbol"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_8abdae0b-1524-45e4-89f5-5cce0fe00d2b" xlink:to="loc_dei_TradingSymbol_058f81c7-d191-48eb-8433-06aa27d56ee0" xlink:type="arc" order="16"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_SecurityExchangeName_d8eb2b4c-ed78-4531-93b1-f73d263599c7" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_SecurityExchangeName"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_8abdae0b-1524-45e4-89f5-5cce0fe00d2b" xlink:to="loc_dei_SecurityExchangeName_d8eb2b4c-ed78-4531-93b1-f73d263599c7" xlink:type="arc" order="17"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityCurrentReportingStatus_fd25b3ba-e6f4-4a78-ab02-0549c6f421a8" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityCurrentReportingStatus"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_8abdae0b-1524-45e4-89f5-5cce0fe00d2b" xlink:to="loc_dei_EntityCurrentReportingStatus_fd25b3ba-e6f4-4a78-ab02-0549c6f421a8" xlink:type="arc" order="18"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityInteractiveDataCurrent_cd52ccdd-b0c7-4a6e-a62c-c52654298baa" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityInteractiveDataCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_8abdae0b-1524-45e4-89f5-5cce0fe00d2b" xlink:to="loc_dei_EntityInteractiveDataCurrent_cd52ccdd-b0c7-4a6e-a62c-c52654298baa" xlink:type="arc" order="19"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityFilerCategory_e54261c4-5599-4f7f-93dc-c33fd272d707" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityFilerCategory"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_8abdae0b-1524-45e4-89f5-5cce0fe00d2b" xlink:to="loc_dei_EntityFilerCategory_e54261c4-5599-4f7f-93dc-c33fd272d707" xlink:type="arc" order="20"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntitySmallBusiness_8c474e38-d5af-4c6b-9ec2-994433beea97" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntitySmallBusiness"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_8abdae0b-1524-45e4-89f5-5cce0fe00d2b" xlink:to="loc_dei_EntitySmallBusiness_8c474e38-d5af-4c6b-9ec2-994433beea97" xlink:type="arc" order="21"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityEmergingGrowthCompany_e643be5a-c495-4d4e-92c0-9b69664007d0" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityEmergingGrowthCompany"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_8abdae0b-1524-45e4-89f5-5cce0fe00d2b" xlink:to="loc_dei_EntityEmergingGrowthCompany_e643be5a-c495-4d4e-92c0-9b69664007d0" xlink:type="arc" order="22"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityShellCompany_d27f4fb6-d7ab-44ed-a49a-0f7a48de8cd2" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityShellCompany"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_8abdae0b-1524-45e4-89f5-5cce0fe00d2b" xlink:to="loc_dei_EntityShellCompany_d27f4fb6-d7ab-44ed-a49a-0f7a48de8cd2" xlink:type="arc" order="23"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityCommonStockSharesOutstanding_b96ba0d2-1514-4b00-b8b0-ae33e902c922" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityCommonStockSharesOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_8abdae0b-1524-45e4-89f5-5cce0fe00d2b" xlink:to="loc_dei_EntityCommonStockSharesOutstanding_b96ba0d2-1514-4b00-b8b0-ae33e902c922" xlink:type="arc" order="24"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityCentralIndexKey_85038373-f999-414a-8008-fa6bb469034b" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityCentralIndexKey"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_8abdae0b-1524-45e4-89f5-5cce0fe00d2b" xlink:to="loc_dei_EntityCentralIndexKey_85038373-f999-414a-8008-fa6bb469034b" xlink:type="arc" order="25"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_CurrentFiscalYearEndDate_076e46f6-d159-42d0-af89-fb720267da27" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_CurrentFiscalYearEndDate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_8abdae0b-1524-45e4-89f5-5cce0fe00d2b" xlink:to="loc_dei_CurrentFiscalYearEndDate_076e46f6-d159-42d0-af89-fb720267da27" xlink:type="arc" order="26"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_AmendmentFlag_5debf4d3-ff7f-4d72-bdd0-4d0ac15f0807" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_AmendmentFlag"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_8abdae0b-1524-45e4-89f5-5cce0fe00d2b" xlink:to="loc_dei_AmendmentFlag_5debf4d3-ff7f-4d72-bdd0-4d0ac15f0807" xlink:type="arc" order="27"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentFiscalYearFocus_c215c302-7a1d-4cc5-9b4e-075a20a41507" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_DocumentFiscalYearFocus"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_8abdae0b-1524-45e4-89f5-5cce0fe00d2b" xlink:to="loc_dei_DocumentFiscalYearFocus_c215c302-7a1d-4cc5-9b4e-075a20a41507" xlink:type="arc" order="28"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentFiscalPeriodFocus_75425883-5703-4178-bbf1-67006e447e0c" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_DocumentFiscalPeriodFocus"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_8abdae0b-1524-45e4-89f5-5cce0fe00d2b" xlink:to="loc_dei_DocumentFiscalPeriodFocus_75425883-5703-4178-bbf1-67006e447e0c" xlink:type="arc" order="29"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.centrusenergy.com/role/CONSOLIDATEDBALANCESHEETS" xlink:type="simple" xlink:href="leu-20240630.xsd#CONSOLIDATEDBALANCESHEETS"/>
  <link:definitionLink xlink:role="http://www.centrusenergy.com/role/CONSOLIDATEDBALANCESHEETS" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_26b7a365-61bc-4b02-9d92-e242ec1ae80c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_66f4f72d-f5cc-4443-8904-49a47602efb2" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_26b7a365-61bc-4b02-9d92-e242ec1ae80c" xlink:to="loc_us-gaap_StatementTable_66f4f72d-f5cc-4443-8904-49a47602efb2" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementClassOfStockAxis_ed9d74c0-165e-4f09-8e0e-6385862aad31" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementClassOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_66f4f72d-f5cc-4443-8904-49a47602efb2" xlink:to="loc_us-gaap_StatementClassOfStockAxis_ed9d74c0-165e-4f09-8e0e-6385862aad31" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_ed9d74c0-165e-4f09-8e0e-6385862aad31_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementClassOfStockAxis_ed9d74c0-165e-4f09-8e0e-6385862aad31" xlink:to="loc_us-gaap_ClassOfStockDomain_ed9d74c0-165e-4f09-8e0e-6385862aad31_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_9fa3fa1a-b038-4bba-bb59-916f7a3626bc" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementClassOfStockAxis_ed9d74c0-165e-4f09-8e0e-6385862aad31" xlink:to="loc_us-gaap_ClassOfStockDomain_9fa3fa1a-b038-4bba-bb59-916f7a3626bc" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonClassAMember_4cf95db3-be60-4cab-8ca0-afa0697d5c6f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommonClassAMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_9fa3fa1a-b038-4bba-bb59-916f7a3626bc" xlink:to="loc_us-gaap_CommonClassAMember_4cf95db3-be60-4cab-8ca0-afa0697d5c6f" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonClassBMember_7606fa29-bcd3-4a5c-82d9-78a056de6784" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommonClassBMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_9fa3fa1a-b038-4bba-bb59-916f7a3626bc" xlink:to="loc_us-gaap_CommonClassBMember_7606fa29-bcd3-4a5c-82d9-78a056de6784" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsAbstract_54d269ad-1ceb-4dd0-ad6b-9ee8807cb033" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AssetsAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_26b7a365-61bc-4b02-9d92-e242ec1ae80c" xlink:to="loc_us-gaap_AssetsAbstract_54d269ad-1ceb-4dd0-ad6b-9ee8807cb033" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsCurrentAbstract_e30500d2-83f2-4251-9375-a63dba2a7323" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AssetsCurrentAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsAbstract_54d269ad-1ceb-4dd0-ad6b-9ee8807cb033" xlink:to="loc_us-gaap_AssetsCurrentAbstract_e30500d2-83f2-4251-9375-a63dba2a7323" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashAndCashEquivalentsAtCarryingValue_75511ff0-00b2-43a9-9ca6-0882a1994fbc" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CashAndCashEquivalentsAtCarryingValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsCurrentAbstract_e30500d2-83f2-4251-9375-a63dba2a7323" xlink:to="loc_us-gaap_CashAndCashEquivalentsAtCarryingValue_75511ff0-00b2-43a9-9ca6-0882a1994fbc" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountsReceivableNetCurrent_b342df5d-93c2-4e62-9f8a-e95a68551e66" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccountsReceivableNetCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsCurrentAbstract_e30500d2-83f2-4251-9375-a63dba2a7323" xlink:to="loc_us-gaap_AccountsReceivableNetCurrent_b342df5d-93c2-4e62-9f8a-e95a68551e66" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InventoryNet_a3cecaa5-a785-40c2-9b3f-dc76d994757f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_InventoryNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsCurrentAbstract_e30500d2-83f2-4251-9375-a63dba2a7323" xlink:to="loc_us-gaap_InventoryNet_a3cecaa5-a785-40c2-9b3f-dc76d994757f" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherDeferredCostsNet_d554234f-a331-488a-a166-06eac3bc0513" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OtherDeferredCostsNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsCurrentAbstract_e30500d2-83f2-4251-9375-a63dba2a7323" xlink:to="loc_us-gaap_OtherDeferredCostsNet_d554234f-a331-488a-a166-06eac3bc0513" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PrepaidExpenseAndOtherAssetsCurrent_2231410c-038b-4059-a951-8946e2b29cd1" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PrepaidExpenseAndOtherAssetsCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsCurrentAbstract_e30500d2-83f2-4251-9375-a63dba2a7323" xlink:to="loc_us-gaap_PrepaidExpenseAndOtherAssetsCurrent_2231410c-038b-4059-a951-8946e2b29cd1" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsCurrent_03d3953f-8bb3-420c-b17f-66333a3e36ca" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AssetsCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsCurrentAbstract_e30500d2-83f2-4251-9375-a63dba2a7323" xlink:to="loc_us-gaap_AssetsCurrent_03d3953f-8bb3-420c-b17f-66333a3e36ca" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentNet_85c48244-1014-4370-bda8-b23a6bae606c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PropertyPlantAndEquipmentNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsAbstract_54d269ad-1ceb-4dd0-ad6b-9ee8807cb033" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentNet_85c48244-1014-4370-bda8-b23a6bae606c" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DepositsAssetsNoncurrent_ea74d908-dbf9-46ff-85a3-a27f2b14b610" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DepositsAssetsNoncurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsAbstract_54d269ad-1ceb-4dd0-ad6b-9ee8807cb033" xlink:to="loc_us-gaap_DepositsAssetsNoncurrent_ea74d908-dbf9-46ff-85a3-a27f2b14b610" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IntangibleAssetsNetExcludingGoodwill_ec052564-3ba4-483d-958a-c985da3041c8" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IntangibleAssetsNetExcludingGoodwill"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsAbstract_54d269ad-1ceb-4dd0-ad6b-9ee8807cb033" xlink:to="loc_us-gaap_IntangibleAssetsNetExcludingGoodwill_ec052564-3ba4-483d-958a-c985da3041c8" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredIncomeTaxAssetsNet_fb4550b8-e0af-494e-8a7f-e66c008fd7ba" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DeferredIncomeTaxAssetsNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsAbstract_54d269ad-1ceb-4dd0-ad6b-9ee8807cb033" xlink:to="loc_us-gaap_DeferredIncomeTaxAssetsNet_fb4550b8-e0af-494e-8a7f-e66c008fd7ba" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherAssetsNoncurrent_68a1be18-d249-46bf-abd4-62b95cfa3087" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OtherAssetsNoncurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsAbstract_54d269ad-1ceb-4dd0-ad6b-9ee8807cb033" xlink:to="loc_us-gaap_OtherAssetsNoncurrent_68a1be18-d249-46bf-abd4-62b95cfa3087" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Assets_d78e074e-a1c1-453c-97f1-892adf065b0e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_Assets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsAbstract_54d269ad-1ceb-4dd0-ad6b-9ee8807cb033" xlink:to="loc_us-gaap_Assets_d78e074e-a1c1-453c-97f1-892adf065b0e" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_0d9fe09a-2530-4f91-a336-4b64faf9c3a8" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LiabilitiesAndStockholdersEquityAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_26b7a365-61bc-4b02-9d92-e242ec1ae80c" xlink:to="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_0d9fe09a-2530-4f91-a336-4b64faf9c3a8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesCurrentAbstract_03517b9b-98c8-4360-be80-ce9b910bdd9e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LiabilitiesCurrentAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_0d9fe09a-2530-4f91-a336-4b64faf9c3a8" xlink:to="loc_us-gaap_LiabilitiesCurrentAbstract_03517b9b-98c8-4360-be80-ce9b910bdd9e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountsPayableAndAccruedLiabilitiesCurrent_4dcab173-3159-4df4-97f0-800c097be766" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccountsPayableAndAccruedLiabilitiesCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesCurrentAbstract_03517b9b-98c8-4360-be80-ce9b910bdd9e" xlink:to="loc_us-gaap_AccountsPayableAndAccruedLiabilitiesCurrent_4dcab173-3159-4df4-97f0-800c097be766" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_AccountsPayableUnderPurchaseAgreements_3d211a73-3551-46c4-a455-52bf0b5b789a" xlink:href="leu-20240630.xsd#leu_AccountsPayableUnderPurchaseAgreements"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesCurrentAbstract_03517b9b-98c8-4360-be80-ce9b910bdd9e" xlink:to="loc_leu_AccountsPayableUnderPurchaseAgreements_3d211a73-3551-46c4-a455-52bf0b5b789a" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_InventoriesOwedToCustomersAndSuppliers_9c379f40-fe03-49d6-a2ae-f70a697b17a8" xlink:href="leu-20240630.xsd#leu_InventoriesOwedToCustomersAndSuppliers"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesCurrentAbstract_03517b9b-98c8-4360-be80-ce9b910bdd9e" xlink:to="loc_leu_InventoriesOwedToCustomersAndSuppliers_9c379f40-fe03-49d6-a2ae-f70a697b17a8" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ContractWithCustomerLiabilityCurrent_70e99f00-dd3a-4652-ae45-96f0a3e0f10f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ContractWithCustomerLiabilityCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesCurrentAbstract_03517b9b-98c8-4360-be80-ce9b910bdd9e" xlink:to="loc_us-gaap_ContractWithCustomerLiabilityCurrent_70e99f00-dd3a-4652-ae45-96f0a3e0f10f" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_ShortTermInventoryLoans_cfc7e95d-48dc-4b07-a952-ebd2325f9d55" xlink:href="leu-20240630.xsd#leu_ShortTermInventoryLoans"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesCurrentAbstract_03517b9b-98c8-4360-be80-ce9b910bdd9e" xlink:to="loc_leu_ShortTermInventoryLoans_cfc7e95d-48dc-4b07-a952-ebd2325f9d55" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtCurrent_5f2c7301-a144-42ab-af74-e7322a2cddfd" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesCurrentAbstract_03517b9b-98c8-4360-be80-ce9b910bdd9e" xlink:to="loc_us-gaap_DebtCurrent_5f2c7301-a144-42ab-af74-e7322a2cddfd" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesCurrent_2eebcca8-8e94-4445-a9e2-a258a4dca105" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LiabilitiesCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesCurrentAbstract_03517b9b-98c8-4360-be80-ce9b910bdd9e" xlink:to="loc_us-gaap_LiabilitiesCurrent_2eebcca8-8e94-4445-a9e2-a258a4dca105" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebt_059d5a48-b9aa-45d2-bcec-f54955f8a9a7" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LongTermDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_0d9fe09a-2530-4f91-a336-4b64faf9c3a8" xlink:to="loc_us-gaap_LongTermDebt_059d5a48-b9aa-45d2-bcec-f54955f8a9a7" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherPostretirementDefinedBenefitPlanLiabilitiesNoncurrent_074ec84a-b78b-4a5c-91e1-65c9f68198fe" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OtherPostretirementDefinedBenefitPlanLiabilitiesNoncurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_0d9fe09a-2530-4f91-a336-4b64faf9c3a8" xlink:to="loc_us-gaap_OtherPostretirementDefinedBenefitPlanLiabilitiesNoncurrent_074ec84a-b78b-4a5c-91e1-65c9f68198fe" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPensionPlanLiabilitiesNoncurrent_7086ed7f-49c0-41ee-8a5b-ec6cecc73603" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DefinedBenefitPensionPlanLiabilitiesNoncurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_0d9fe09a-2530-4f91-a336-4b64faf9c3a8" xlink:to="loc_us-gaap_DefinedBenefitPensionPlanLiabilitiesNoncurrent_7086ed7f-49c0-41ee-8a5b-ec6cecc73603" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ContractWithCustomerLiabilityNoncurrent_c8b319c8-fbd4-4fe1-b7fd-c6e8fea1ed08" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ContractWithCustomerLiabilityNoncurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_0d9fe09a-2530-4f91-a336-4b64faf9c3a8" xlink:to="loc_us-gaap_ContractWithCustomerLiabilityNoncurrent_c8b319c8-fbd4-4fe1-b7fd-c6e8fea1ed08" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_LongTermInventoryLoans_eb368603-bf58-40d2-9f23-ae431eafc3df" xlink:href="leu-20240630.xsd#leu_LongTermInventoryLoans"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_0d9fe09a-2530-4f91-a336-4b64faf9c3a8" xlink:to="loc_leu_LongTermInventoryLoans_eb368603-bf58-40d2-9f23-ae431eafc3df" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherLiabilitiesNoncurrent_235ab834-9e97-494c-9273-076c69e070bf" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OtherLiabilitiesNoncurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_0d9fe09a-2530-4f91-a336-4b64faf9c3a8" xlink:to="loc_us-gaap_OtherLiabilitiesNoncurrent_235ab834-9e97-494c-9273-076c69e070bf" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Liabilities_3f28fc07-d434-4a8e-b7cf-7a1f3080388a" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_Liabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_0d9fe09a-2530-4f91-a336-4b64faf9c3a8" xlink:to="loc_us-gaap_Liabilities_3f28fc07-d434-4a8e-b7cf-7a1f3080388a" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommitmentsAndContingencies_ff0171a9-4b91-421b-9d6c-13d2069291f2" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommitmentsAndContingencies"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_0d9fe09a-2530-4f91-a336-4b64faf9c3a8" xlink:to="loc_us-gaap_CommitmentsAndContingencies_ff0171a9-4b91-421b-9d6c-13d2069291f2" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquityAbstract_c8e75428-0db5-40dc-beb8-7c415633847c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StockholdersEquityAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_0d9fe09a-2530-4f91-a336-4b64faf9c3a8" xlink:to="loc_us-gaap_StockholdersEquityAbstract_c8e75428-0db5-40dc-beb8-7c415633847c" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockValue_1179ffb5-4405-4986-8830-c76a165512cc" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PreferredStockValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StockholdersEquityAbstract_c8e75428-0db5-40dc-beb8-7c415633847c" xlink:to="loc_us-gaap_PreferredStockValue_1179ffb5-4405-4986-8830-c76a165512cc" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockSharesIssued_9d14c669-f23c-483d-b038-c4c7b3aba0bb" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommonStockSharesIssued"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StockholdersEquityAbstract_c8e75428-0db5-40dc-beb8-7c415633847c" xlink:to="loc_us-gaap_CommonStockSharesIssued_9d14c669-f23c-483d-b038-c4c7b3aba0bb" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockValue_09b2b22a-6e2a-4e89-8ae8-af34a19639c7" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommonStockValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StockholdersEquityAbstract_c8e75428-0db5-40dc-beb8-7c415633847c" xlink:to="loc_us-gaap_CommonStockValue_09b2b22a-6e2a-4e89-8ae8-af34a19639c7" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdditionalPaidInCapital_2f7ba2aa-8031-4357-82b0-662224ec0dc4" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AdditionalPaidInCapital"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StockholdersEquityAbstract_c8e75428-0db5-40dc-beb8-7c415633847c" xlink:to="loc_us-gaap_AdditionalPaidInCapital_2f7ba2aa-8031-4357-82b0-662224ec0dc4" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetainedEarningsAccumulatedDeficit_de7100db-a1ee-40cb-97e6-e57835059605" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RetainedEarningsAccumulatedDeficit"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StockholdersEquityAbstract_c8e75428-0db5-40dc-beb8-7c415633847c" xlink:to="loc_us-gaap_RetainedEarningsAccumulatedDeficit_de7100db-a1ee-40cb-97e6-e57835059605" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossNetOfTax_29a6a303-1141-4e3b-9b0d-6063cb64e99b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeLossNetOfTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StockholdersEquityAbstract_c8e75428-0db5-40dc-beb8-7c415633847c" xlink:to="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossNetOfTax_29a6a303-1141-4e3b-9b0d-6063cb64e99b" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_aefdc9dd-df80-4f52-a57c-abbc73347de1" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StockholdersEquity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StockholdersEquityAbstract_c8e75428-0db5-40dc-beb8-7c415633847c" xlink:to="loc_us-gaap_StockholdersEquity_aefdc9dd-df80-4f52-a57c-abbc73347de1" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesAndStockholdersEquity_44fbf17a-50ff-4f8d-b822-92580fa4c0fa" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LiabilitiesAndStockholdersEquity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_0d9fe09a-2530-4f91-a336-4b64faf9c3a8" xlink:to="loc_us-gaap_LiabilitiesAndStockholdersEquity_44fbf17a-50ff-4f8d-b822-92580fa4c0fa" xlink:type="arc" order="11"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.centrusenergy.com/role/CONSOLIDATEDBALANCESHEETSParenthetical" xlink:type="simple" xlink:href="leu-20240630.xsd#CONSOLIDATEDBALANCESHEETSParenthetical"/>
  <link:definitionLink xlink:role="http://www.centrusenergy.com/role/CONSOLIDATEDBALANCESHEETSParenthetical" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_4507c6e4-02d1-40fa-a589-410bc355285e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_3f0f33f0-dc70-4d53-ab6b-eb44868c9b52" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_4507c6e4-02d1-40fa-a589-410bc355285e" xlink:to="loc_us-gaap_StatementTable_3f0f33f0-dc70-4d53-ab6b-eb44868c9b52" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementClassOfStockAxis_4c024a95-5bfb-4e71-9b23-54f58eda884f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementClassOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_3f0f33f0-dc70-4d53-ab6b-eb44868c9b52" xlink:to="loc_us-gaap_StatementClassOfStockAxis_4c024a95-5bfb-4e71-9b23-54f58eda884f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_4c024a95-5bfb-4e71-9b23-54f58eda884f_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementClassOfStockAxis_4c024a95-5bfb-4e71-9b23-54f58eda884f" xlink:to="loc_us-gaap_ClassOfStockDomain_4c024a95-5bfb-4e71-9b23-54f58eda884f_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_b9193155-dac8-4b4d-9e86-de7f35342060" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementClassOfStockAxis_4c024a95-5bfb-4e71-9b23-54f58eda884f" xlink:to="loc_us-gaap_ClassOfStockDomain_b9193155-dac8-4b4d-9e86-de7f35342060" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonClassAMember_9342bdca-1a49-4855-9039-6baee68e947d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommonClassAMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_b9193155-dac8-4b4d-9e86-de7f35342060" xlink:to="loc_us-gaap_CommonClassAMember_9342bdca-1a49-4855-9039-6baee68e947d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonClassBMember_fff3f558-64e5-4e1b-9aba-8eec6dc202d3" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommonClassBMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_b9193155-dac8-4b4d-9e86-de7f35342060" xlink:to="loc_us-gaap_CommonClassBMember_fff3f558-64e5-4e1b-9aba-8eec6dc202d3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockParOrStatedValuePerShare_8743db08-aa56-4a25-868e-a6538c9850ca" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PreferredStockParOrStatedValuePerShare"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_4507c6e4-02d1-40fa-a589-410bc355285e" xlink:to="loc_us-gaap_PreferredStockParOrStatedValuePerShare_8743db08-aa56-4a25-868e-a6538c9850ca" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockSharesAuthorized_b3d848f8-8bf8-4f00-b860-9a036679f281" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PreferredStockSharesAuthorized"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_4507c6e4-02d1-40fa-a589-410bc355285e" xlink:to="loc_us-gaap_PreferredStockSharesAuthorized_b3d848f8-8bf8-4f00-b860-9a036679f281" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockParOrStatedValuePerShare_bfdf8ad7-6bcb-4d96-bfdc-d6052ea4385b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommonStockParOrStatedValuePerShare"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_4507c6e4-02d1-40fa-a589-410bc355285e" xlink:to="loc_us-gaap_CommonStockParOrStatedValuePerShare_bfdf8ad7-6bcb-4d96-bfdc-d6052ea4385b" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockSharesOutstanding_20e10d9c-6719-4a93-9cb2-106e14026517" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommonStockSharesOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_4507c6e4-02d1-40fa-a589-410bc355285e" xlink:to="loc_us-gaap_CommonStockSharesOutstanding_20e10d9c-6719-4a93-9cb2-106e14026517" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockSharesAuthorized_56e38008-9bdf-4b1f-8615-44a969ab59f0" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommonStockSharesAuthorized"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_4507c6e4-02d1-40fa-a589-410bc355285e" xlink:to="loc_us-gaap_CommonStockSharesAuthorized_56e38008-9bdf-4b1f-8615-44a969ab59f0" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockSharesIssued_1d0440d0-2a2d-4260-b5f9-c7a0242db15e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommonStockSharesIssued"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_4507c6e4-02d1-40fa-a589-410bc355285e" xlink:to="loc_us-gaap_CommonStockSharesIssued_1d0440d0-2a2d-4260-b5f9-c7a0242db15e" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment_84d8b276-10ad-4053-a4e9-62753efaaf33" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_4507c6e4-02d1-40fa-a589-410bc355285e" xlink:to="loc_us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment_84d8b276-10ad-4053-a4e9-62753efaaf33" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment_6657be96-ebd5-4d7f-8af6-6ce801787e6d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.centrusenergy.com/role/CONSOLIDATEDSTATEMENTSOFOPERATIONS" xlink:type="simple" xlink:href="leu-20240630.xsd#CONSOLIDATEDSTATEMENTSOFOPERATIONS"/>
  <link:definitionLink xlink:role="http://www.centrusenergy.com/role/CONSOLIDATEDSTATEMENTSOFOPERATIONS" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_e025be79-134c-4941-9ca9-9c5db217f5b2" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_2c8c87f4-104f-47fc-bf8d-62922f123df7" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_e025be79-134c-4941-9ca9-9c5db217f5b2" xlink:to="loc_us-gaap_StatementTable_2c8c87f4-104f-47fc-bf8d-62922f123df7" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductOrServiceAxis_b4cdf6c8-3a78-42c4-bdb6-517fc50892be" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_ProductOrServiceAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_2c8c87f4-104f-47fc-bf8d-62922f123df7" xlink:to="loc_srt_ProductOrServiceAxis_b4cdf6c8-3a78-42c4-bdb6-517fc50892be" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_b4cdf6c8-3a78-42c4-bdb6-517fc50892be_default" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_ProductOrServiceAxis_b4cdf6c8-3a78-42c4-bdb6-517fc50892be" xlink:to="loc_srt_ProductsAndServicesDomain_b4cdf6c8-3a78-42c4-bdb6-517fc50892be_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_ce7ffb69-4be0-45b2-ba4b-fd86f4618d40" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_ProductOrServiceAxis_b4cdf6c8-3a78-42c4-bdb6-517fc50892be" xlink:to="loc_srt_ProductsAndServicesDomain_ce7ffb69-4be0-45b2-ba4b-fd86f4618d40" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProductMember_8bcbbde0-b412-47a0-ad58-77bf9cd4d5e5" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ProductMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_ce7ffb69-4be0-45b2-ba4b-fd86f4618d40" xlink:to="loc_us-gaap_ProductMember_8bcbbde0-b412-47a0-ad58-77bf9cd4d5e5" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_UraniumMember_e79a2249-92c8-4801-8dda-12c6d8d37c1e" xlink:href="leu-20240630.xsd#leu_UraniumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ProductMember_8bcbbde0-b412-47a0-ad58-77bf9cd4d5e5" xlink:to="loc_leu_UraniumMember_e79a2249-92c8-4801-8dda-12c6d8d37c1e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_SeparativeWorkUnitsMember_d469b879-d854-4db8-9173-bb212a2d6694" xlink:href="leu-20240630.xsd#leu_SeparativeWorkUnitsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ProductMember_8bcbbde0-b412-47a0-ad58-77bf9cd4d5e5" xlink:to="loc_leu_SeparativeWorkUnitsMember_d469b879-d854-4db8-9173-bb212a2d6694" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ServiceMember_a49d10f5-db95-4169-9bf6-7682b5550134" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ServiceMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_ce7ffb69-4be0-45b2-ba4b-fd86f4618d40" xlink:to="loc_us-gaap_ServiceMember_a49d10f5-db95-4169-9bf6-7682b5550134" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_adc4f4f3-1b76-45b8-b95d-49c00f00ed61" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_e025be79-134c-4941-9ca9-9c5db217f5b2" xlink:to="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_adc4f4f3-1b76-45b8-b95d-49c00f00ed61" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CostOfGoodsAndServicesSold_51fc4174-f2d0-4955-8be8-638b439bc35a" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CostOfGoodsAndServicesSold"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_e025be79-134c-4941-9ca9-9c5db217f5b2" xlink:to="loc_us-gaap_CostOfGoodsAndServicesSold_51fc4174-f2d0-4955-8be8-638b439bc35a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GrossProfit_d297c650-f496-4d07-a61c-62451a2d1994" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_GrossProfit"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_e025be79-134c-4941-9ca9-9c5db217f5b2" xlink:to="loc_us-gaap_GrossProfit_d297c650-f496-4d07-a61c-62451a2d1994" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ResearchAndDevelopmentExpense_dd187dea-5c68-493e-8410-7c4bf398c5a4" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ResearchAndDevelopmentExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_e025be79-134c-4941-9ca9-9c5db217f5b2" xlink:to="loc_us-gaap_ResearchAndDevelopmentExpense_dd187dea-5c68-493e-8410-7c4bf398c5a4" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SellingGeneralAndAdministrativeExpense_c0342a5e-6dfb-467f-b8fe-7ece48a57ef4" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SellingGeneralAndAdministrativeExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_e025be79-134c-4941-9ca9-9c5db217f5b2" xlink:to="loc_us-gaap_SellingGeneralAndAdministrativeExpense_c0342a5e-6dfb-467f-b8fe-7ece48a57ef4" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AmortizationOfIntangibleAssets_c816270e-cabd-4690-acf8-3e69d9dc4bf3" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AmortizationOfIntangibleAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_e025be79-134c-4941-9ca9-9c5db217f5b2" xlink:to="loc_us-gaap_AmortizationOfIntangibleAssets_c816270e-cabd-4690-acf8-3e69d9dc4bf3" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestructuringCharges_5b924ff1-d0bd-4594-98e2-0704d203a0dd" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RestructuringCharges"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_e025be79-134c-4941-9ca9-9c5db217f5b2" xlink:to="loc_us-gaap_RestructuringCharges_5b924ff1-d0bd-4594-98e2-0704d203a0dd" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingIncomeLoss_0a7f8078-9026-48d4-b1ea-12324ab164b6" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OperatingIncomeLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_e025be79-134c-4941-9ca9-9c5db217f5b2" xlink:to="loc_us-gaap_OperatingIncomeLoss_0a7f8078-9026-48d4-b1ea-12324ab164b6" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetPeriodicDefinedBenefitsExpenseReversalOfExpenseExcludingServiceCostComponent_75e41cb9-f5a1-41d0-8b4f-14eb74b641bb" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NetPeriodicDefinedBenefitsExpenseReversalOfExpenseExcludingServiceCostComponent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_e025be79-134c-4941-9ca9-9c5db217f5b2" xlink:to="loc_us-gaap_NetPeriodicDefinedBenefitsExpenseReversalOfExpenseExcludingServiceCostComponent_75e41cb9-f5a1-41d0-8b4f-14eb74b641bb" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestExpenseDebt_c20a1a2d-de6d-4fca-9376-6e1b66d7bd01" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_InterestExpenseDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_e025be79-134c-4941-9ca9-9c5db217f5b2" xlink:to="loc_us-gaap_InterestExpenseDebt_c20a1a2d-de6d-4fca-9376-6e1b66d7bd01" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InvestmentIncomeInterest_5be150c5-b2f9-42b4-8d36-6885f74456cd" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_InvestmentIncomeInterest"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_e025be79-134c-4941-9ca9-9c5db217f5b2" xlink:to="loc_us-gaap_InvestmentIncomeInterest_5be150c5-b2f9-42b4-8d36-6885f74456cd" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherNonoperatingIncome_a5caecf4-6679-4eba-833a-4a05b3fe12fb" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OtherNonoperatingIncome"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_e025be79-134c-4941-9ca9-9c5db217f5b2" xlink:to="loc_us-gaap_OtherNonoperatingIncome_a5caecf4-6679-4eba-833a-4a05b3fe12fb" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_76d9d3d3-f93c-494f-ac5a-56e0d94f7af8" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_e025be79-134c-4941-9ca9-9c5db217f5b2" xlink:to="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_76d9d3d3-f93c-494f-ac5a-56e0d94f7af8" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxExpenseBenefit_c32f6b52-bc95-47b3-bda7-3b8dbbe74636" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncomeTaxExpenseBenefit"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_e025be79-134c-4941-9ca9-9c5db217f5b2" xlink:to="loc_us-gaap_IncomeTaxExpenseBenefit_c32f6b52-bc95-47b3-bda7-3b8dbbe74636" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_68a044d4-b165-4bb8-a860-3e9f131c4507" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NetIncomeLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_e025be79-134c-4941-9ca9-9c5db217f5b2" xlink:to="loc_us-gaap_NetIncomeLoss_68a044d4-b165-4bb8-a860-3e9f131c4507" xlink:type="arc" order="14"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EarningsPerShareBasic_9c982812-182f-4d68-b0ed-5bbc460750bb" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EarningsPerShareBasic"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_e025be79-134c-4941-9ca9-9c5db217f5b2" xlink:to="loc_us-gaap_EarningsPerShareBasic_9c982812-182f-4d68-b0ed-5bbc460750bb" xlink:type="arc" order="15"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EarningsPerShareDiluted_95df98b4-eb69-402b-953f-947a3a5106ab" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EarningsPerShareDiluted"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_e025be79-134c-4941-9ca9-9c5db217f5b2" xlink:to="loc_us-gaap_EarningsPerShareDiluted_95df98b4-eb69-402b-953f-947a3a5106ab" xlink:type="arc" order="16"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_WeightedAverageNumberOfSharesOutstandingAbstract_fa728b20-897d-4270-a4b3-7d46bc1e1bc2" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_WeightedAverageNumberOfSharesOutstandingAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_e025be79-134c-4941-9ca9-9c5db217f5b2" xlink:to="loc_us-gaap_WeightedAverageNumberOfSharesOutstandingAbstract_fa728b20-897d-4270-a4b3-7d46bc1e1bc2" xlink:type="arc" order="17"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_WeightedAverageNumberOfSharesOutstandingBasic_5eeffc33-ed67-41d1-bb90-2e1ff4e61dd4" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_WeightedAverageNumberOfSharesOutstandingBasic"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_WeightedAverageNumberOfSharesOutstandingAbstract_fa728b20-897d-4270-a4b3-7d46bc1e1bc2" xlink:to="loc_us-gaap_WeightedAverageNumberOfSharesOutstandingBasic_5eeffc33-ed67-41d1-bb90-2e1ff4e61dd4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_WeightedAverageNumberOfDilutedSharesOutstanding_503ffa91-46d7-4e05-9b58-ababcb2aacc9" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_WeightedAverageNumberOfDilutedSharesOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_WeightedAverageNumberOfSharesOutstandingAbstract_fa728b20-897d-4270-a4b3-7d46bc1e1bc2" xlink:to="loc_us-gaap_WeightedAverageNumberOfDilutedSharesOutstanding_503ffa91-46d7-4e05-9b58-ababcb2aacc9" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.centrusenergy.com/role/CONSOLIDATEDSTATEMENTSOFCASHFLOWS" xlink:type="simple" xlink:href="leu-20240630.xsd#CONSOLIDATEDSTATEMENTSOFCASHFLOWS"/>
  <link:definitionLink xlink:role="http://www.centrusenergy.com/role/CONSOLIDATEDSTATEMENTSOFCASHFLOWS" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_0e001e46-4975-4e59-8cb8-52042fa73a91" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_9fa231bb-151b-4b5a-97b2-e38d2168c91e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_0e001e46-4975-4e59-8cb8-52042fa73a91" xlink:to="loc_us-gaap_StatementTable_9fa231bb-151b-4b5a-97b2-e38d2168c91e" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherSignificantNoncashTransactionsByUniqueDescriptionAxis_0c5f039d-cce2-4a1d-81fe-6c99433a8bb0" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OtherSignificantNoncashTransactionsByUniqueDescriptionAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_9fa231bb-151b-4b5a-97b2-e38d2168c91e" xlink:to="loc_us-gaap_OtherSignificantNoncashTransactionsByUniqueDescriptionAxis_0c5f039d-cce2-4a1d-81fe-6c99433a8bb0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherSignificantNoncashTransactionNameDomain_0c5f039d-cce2-4a1d-81fe-6c99433a8bb0_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OtherSignificantNoncashTransactionNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_OtherSignificantNoncashTransactionsByUniqueDescriptionAxis_0c5f039d-cce2-4a1d-81fe-6c99433a8bb0" xlink:to="loc_us-gaap_OtherSignificantNoncashTransactionNameDomain_0c5f039d-cce2-4a1d-81fe-6c99433a8bb0_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherSignificantNoncashTransactionNameDomain_0867a3df-9150-4c5f-a9cf-14738728e6e8" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OtherSignificantNoncashTransactionNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_OtherSignificantNoncashTransactionsByUniqueDescriptionAxis_0c5f039d-cce2-4a1d-81fe-6c99433a8bb0" xlink:to="loc_us-gaap_OtherSignificantNoncashTransactionNameDomain_0867a3df-9150-4c5f-a9cf-14738728e6e8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_ReclassificationOfStockBasedCompensationLiabilityToEquityMember_ba6197e2-2ccc-4445-8d09-64996e5aa24c" xlink:href="leu-20240630.xsd#leu_ReclassificationOfStockBasedCompensationLiabilityToEquityMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OtherSignificantNoncashTransactionNameDomain_0867a3df-9150-4c5f-a9cf-14738728e6e8" xlink:to="loc_leu_ReclassificationOfStockBasedCompensationLiabilityToEquityMember_ba6197e2-2ccc-4445-8d09-64996e5aa24c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_PropertyPlantAndEquipmentIncludedInAccountsPayableAndAccruedLiabilitiesMember_fb2190b7-2770-4431-bee1-aa6bb3c01b00" xlink:href="leu-20240630.xsd#leu_PropertyPlantAndEquipmentIncludedInAccountsPayableAndAccruedLiabilitiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OtherSignificantNoncashTransactionNameDomain_0867a3df-9150-4c5f-a9cf-14738728e6e8" xlink:to="loc_leu_PropertyPlantAndEquipmentIncludedInAccountsPayableAndAccruedLiabilitiesMember_fb2190b7-2770-4431-bee1-aa6bb3c01b00" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_AdjustmentToRightOfUseLeaseDueToModificationDomain_1f1b3cde-ebb9-4314-bc79-78a210833606" xlink:href="leu-20240630.xsd#leu_AdjustmentToRightOfUseLeaseDueToModificationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OtherSignificantNoncashTransactionNameDomain_0867a3df-9150-4c5f-a9cf-14738728e6e8" xlink:to="loc_leu_AdjustmentToRightOfUseLeaseDueToModificationDomain_1f1b3cde-ebb9-4314-bc79-78a210833606" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_EquityIssuanceCostsIncludedInAccountsPayableAndAccruedLiabilitiesMember_e4c31a54-5a19-4631-a8c8-a05d6437aa0d" xlink:href="leu-20240630.xsd#leu_EquityIssuanceCostsIncludedInAccountsPayableAndAccruedLiabilitiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OtherSignificantNoncashTransactionNameDomain_0867a3df-9150-4c5f-a9cf-14738728e6e8" xlink:to="loc_leu_EquityIssuanceCostsIncludedInAccountsPayableAndAccruedLiabilitiesMember_e4c31a54-5a19-4631-a8c8-a05d6437aa0d" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_SharesWithheldForEmployeeTaxesMember_a32e22b4-3f20-457b-902b-4c58af94eb2c" xlink:href="leu-20240630.xsd#leu_SharesWithheldForEmployeeTaxesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OtherSignificantNoncashTransactionNameDomain_0867a3df-9150-4c5f-a9cf-14738728e6e8" xlink:to="loc_leu_SharesWithheldForEmployeeTaxesMember_a32e22b4-3f20-457b-902b-4c58af94eb2c" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_CommonStockIssuanceProceedsInAccountsReceivableDomain_b69540c1-530f-4aec-8908-eab5952c263f" xlink:href="leu-20240630.xsd#leu_CommonStockIssuanceProceedsInAccountsReceivableDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OtherSignificantNoncashTransactionNameDomain_0867a3df-9150-4c5f-a9cf-14738728e6e8" xlink:to="loc_leu_CommonStockIssuanceProceedsInAccountsReceivableDomain_b69540c1-530f-4aec-8908-eab5952c263f" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInOperatingActivitiesContinuingOperationsAbstract_dbb287d7-7cfa-4547-b4aa-fe20b6bd0a40" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NetCashProvidedByUsedInOperatingActivitiesContinuingOperationsAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_0e001e46-4975-4e59-8cb8-52042fa73a91" xlink:to="loc_us-gaap_NetCashProvidedByUsedInOperatingActivitiesContinuingOperationsAbstract_dbb287d7-7cfa-4547-b4aa-fe20b6bd0a40" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_1ef2bd17-5bc1-4213-805b-1af59353df3d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NetIncomeLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivitiesContinuingOperationsAbstract_dbb287d7-7cfa-4547-b4aa-fe20b6bd0a40" xlink:to="loc_us-gaap_NetIncomeLoss_1ef2bd17-5bc1-4213-805b-1af59353df3d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract_1a3f4c4a-489b-45df-94c8-6aaa6b5543b8" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivitiesContinuingOperationsAbstract_dbb287d7-7cfa-4547-b4aa-fe20b6bd0a40" xlink:to="loc_us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract_1a3f4c4a-489b-45df-94c8-6aaa6b5543b8" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DepreciationDepletionAndAmortization_549b5651-85db-40c9-a6b6-3392781ffa76" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DepreciationDepletionAndAmortization"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract_1a3f4c4a-489b-45df-94c8-6aaa6b5543b8" xlink:to="loc_us-gaap_DepreciationDepletionAndAmortization_549b5651-85db-40c9-a6b6-3392781ffa76" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_ProvisionForLossOnContractsUtilized_39ca34d6-d631-405e-b71f-11df49034721" xlink:href="leu-20240630.xsd#leu_ProvisionForLossOnContractsUtilized"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract_1a3f4c4a-489b-45df-94c8-6aaa6b5543b8" xlink:to="loc_leu_ProvisionForLossOnContractsUtilized_39ca34d6-d631-405e-b71f-11df49034721" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInDeferredIncomeTaxes_6b5fbe9f-cfd7-4a10-b2d4-a5cf0fdaedab" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncreaseDecreaseInDeferredIncomeTaxes"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract_1a3f4c4a-489b-45df-94c8-6aaa6b5543b8" xlink:to="loc_us-gaap_IncreaseDecreaseInDeferredIncomeTaxes_6b5fbe9f-cfd7-4a10-b2d4-a5cf0fdaedab" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanActuarialGainLoss_09923ff2-39c6-4ffa-9305-7031e1fa2569" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DefinedBenefitPlanActuarialGainLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract_1a3f4c4a-489b-45df-94c8-6aaa6b5543b8" xlink:to="loc_us-gaap_DefinedBenefitPlanActuarialGainLoss_09923ff2-39c6-4ffa-9305-7031e1fa2569" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_EquityRelatedCompensation_d4fbdc98-0959-40d0-9033-0220fbb91c19" xlink:href="leu-20240630.xsd#leu_EquityRelatedCompensation"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract_1a3f4c4a-489b-45df-94c8-6aaa6b5543b8" xlink:to="loc_leu_EquityRelatedCompensation_d4fbdc98-0959-40d0-9033-0220fbb91c19" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_RevaluationOfInventoryBorrowing_b293749d-858e-4083-9ab9-6784270cd0e4" xlink:href="leu-20240630.xsd#leu_RevaluationOfInventoryBorrowing"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract_1a3f4c4a-489b-45df-94c8-6aaa6b5543b8" xlink:to="loc_leu_RevaluationOfInventoryBorrowing_b293749d-858e-4083-9ab9-6784270cd0e4" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_OtherReconcilingAdjustmentsNet_f9b99297-e595-4ad9-948e-07e779ddf49d" xlink:href="leu-20240630.xsd#leu_OtherReconcilingAdjustmentsNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract_1a3f4c4a-489b-45df-94c8-6aaa6b5543b8" xlink:to="loc_leu_OtherReconcilingAdjustmentsNet_f9b99297-e595-4ad9-948e-07e779ddf49d" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInOperatingLiabilitiesAbstract_05fa73a8-a540-487d-addf-49bf5da988fc" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncreaseDecreaseInOperatingLiabilitiesAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract_1a3f4c4a-489b-45df-94c8-6aaa6b5543b8" xlink:to="loc_us-gaap_IncreaseDecreaseInOperatingLiabilitiesAbstract_05fa73a8-a540-487d-addf-49bf5da988fc" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInAccountsReceivable_07a36226-11ca-428a-a528-808ea3d00fec" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncreaseDecreaseInAccountsReceivable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInOperatingLiabilitiesAbstract_05fa73a8-a540-487d-addf-49bf5da988fc" xlink:to="loc_us-gaap_IncreaseDecreaseInAccountsReceivable_07a36226-11ca-428a-a528-808ea3d00fec" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInInventories_e63edf74-fc0d-47e3-9275-010094747272" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncreaseDecreaseInInventories"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInOperatingLiabilitiesAbstract_05fa73a8-a540-487d-addf-49bf5da988fc" xlink:to="loc_us-gaap_IncreaseDecreaseInInventories_e63edf74-fc0d-47e3-9275-010094747272" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_IncreaseDecreaseInInventoriesOwedToSuppliers_92fc4dd6-b55c-49f4-bdcc-d79331235e4a" xlink:href="leu-20240630.xsd#leu_IncreaseDecreaseInInventoriesOwedToSuppliers"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInOperatingLiabilitiesAbstract_05fa73a8-a540-487d-addf-49bf5da988fc" xlink:to="loc_leu_IncreaseDecreaseInInventoriesOwedToSuppliers_92fc4dd6-b55c-49f4-bdcc-d79331235e4a" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInPrepaidExpensesOther_f9595030-8349-4e53-a3ee-0d502c596898" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncreaseDecreaseInPrepaidExpensesOther"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInOperatingLiabilitiesAbstract_05fa73a8-a540-487d-addf-49bf5da988fc" xlink:to="loc_us-gaap_IncreaseDecreaseInPrepaidExpensesOther_f9595030-8349-4e53-a3ee-0d502c596898" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInAccountsPayableAndAccruedLiabilities_24a90a0a-7626-4cd0-94db-5255d4d758a6" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncreaseDecreaseInAccountsPayableAndAccruedLiabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInOperatingLiabilitiesAbstract_05fa73a8-a540-487d-addf-49bf5da988fc" xlink:to="loc_us-gaap_IncreaseDecreaseInAccountsPayableAndAccruedLiabilities_24a90a0a-7626-4cd0-94db-5255d4d758a6" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInAccountsPayableTrade_0a11639f-93cc-4d7b-89d0-1c1c61c5b077" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncreaseDecreaseInAccountsPayableTrade"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInOperatingLiabilitiesAbstract_05fa73a8-a540-487d-addf-49bf5da988fc" xlink:to="loc_us-gaap_IncreaseDecreaseInAccountsPayableTrade_0a11639f-93cc-4d7b-89d0-1c1c61c5b077" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_IncreaseDecreaseinDeferredRevenueNetofDeferredCosts_a0b62925-9536-4130-9380-7ddcc04c6d36" xlink:href="leu-20240630.xsd#leu_IncreaseDecreaseinDeferredRevenueNetofDeferredCosts"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInOperatingLiabilitiesAbstract_05fa73a8-a540-487d-addf-49bf5da988fc" xlink:to="loc_leu_IncreaseDecreaseinDeferredRevenueNetofDeferredCosts_a0b62925-9536-4130-9380-7ddcc04c6d36" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInPensionPlanObligations_04d4380f-119d-4769-afae-4b204585db63" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncreaseDecreaseInPensionPlanObligations"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInOperatingLiabilitiesAbstract_05fa73a8-a540-487d-addf-49bf5da988fc" xlink:to="loc_us-gaap_IncreaseDecreaseInPensionPlanObligations_04d4380f-119d-4769-afae-4b204585db63" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInOtherOperatingCapitalNet_5e078ad1-681e-46c2-adf9-6dfdc0cfe77d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncreaseDecreaseInOtherOperatingCapitalNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInOperatingLiabilitiesAbstract_05fa73a8-a540-487d-addf-49bf5da988fc" xlink:to="loc_us-gaap_IncreaseDecreaseInOtherOperatingCapitalNet_5e078ad1-681e-46c2-adf9-6dfdc0cfe77d" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_15e9fcbc-2603-4082-8f98-8f483dee2288" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NetCashProvidedByUsedInOperatingActivities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivitiesContinuingOperationsAbstract_dbb287d7-7cfa-4547-b4aa-fe20b6bd0a40" xlink:to="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_15e9fcbc-2603-4082-8f98-8f483dee2288" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInInvestingActivitiesAbstract_a9ced0d8-5b95-485e-ad8a-d8dac7f21670" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NetCashProvidedByUsedInInvestingActivitiesAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_0e001e46-4975-4e59-8cb8-52042fa73a91" xlink:to="loc_us-gaap_NetCashProvidedByUsedInInvestingActivitiesAbstract_a9ced0d8-5b95-485e-ad8a-d8dac7f21670" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsToAcquirePropertyPlantAndEquipment_3c6c1f01-5831-4d17-b942-f21fe74933fd" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PaymentsToAcquirePropertyPlantAndEquipment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivitiesAbstract_a9ced0d8-5b95-485e-ad8a-d8dac7f21670" xlink:to="loc_us-gaap_PaymentsToAcquirePropertyPlantAndEquipment_3c6c1f01-5831-4d17-b942-f21fe74933fd" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_e6f27796-9b17-48e1-a871-6832358ebd9e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NetCashProvidedByUsedInInvestingActivities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivitiesAbstract_a9ced0d8-5b95-485e-ad8a-d8dac7f21670" xlink:to="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_e6f27796-9b17-48e1-a871-6832358ebd9e" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract_897561d7-3190-48fe-b9a7-d475c3b2abaa" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_0e001e46-4975-4e59-8cb8-52042fa73a91" xlink:to="loc_us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract_897561d7-3190-48fe-b9a7-d475c3b2abaa" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromIssuanceOfCommonStock_4ccf446f-1f20-4827-954f-061d0160898e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ProceedsFromIssuanceOfCommonStock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract_897561d7-3190-48fe-b9a7-d475c3b2abaa" xlink:to="loc_us-gaap_ProceedsFromIssuanceOfCommonStock_4ccf446f-1f20-4827-954f-061d0160898e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RepaymentsOfDebt_8afb015c-ecab-4ecc-8a79-68e16b2d4d0e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RepaymentsOfDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract_897561d7-3190-48fe-b9a7-d475c3b2abaa" xlink:to="loc_us-gaap_RepaymentsOfDebt_8afb015c-ecab-4ecc-8a79-68e16b2d4d0e" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsRelatedToTaxWithholdingForShareBasedCompensation_6798aef2-7a46-4592-a0dd-a4518929b346" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PaymentsRelatedToTaxWithholdingForShareBasedCompensation"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract_897561d7-3190-48fe-b9a7-d475c3b2abaa" xlink:to="loc_us-gaap_PaymentsRelatedToTaxWithholdingForShareBasedCompensation_6798aef2-7a46-4592-a0dd-a4518929b346" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromPaymentsForOtherFinancingActivities_47e7f62b-82af-47b9-9627-de2a7ccf45c1" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ProceedsFromPaymentsForOtherFinancingActivities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract_897561d7-3190-48fe-b9a7-d475c3b2abaa" xlink:to="loc_us-gaap_ProceedsFromPaymentsForOtherFinancingActivities_47e7f62b-82af-47b9-9627-de2a7ccf45c1" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromStockOptionsExercised_6c152316-c3c3-4e6c-98ad-9126c23d6581" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ProceedsFromStockOptionsExercised"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract_897561d7-3190-48fe-b9a7-d475c3b2abaa" xlink:to="loc_us-gaap_ProceedsFromStockOptionsExercised_6c152316-c3c3-4e6c-98ad-9126c23d6581" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_1d70c629-b2a6-44d2-aee4-95632b147af1" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NetCashProvidedByUsedInFinancingActivities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract_897561d7-3190-48fe-b9a7-d475c3b2abaa" xlink:to="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_1d70c629-b2a6-44d2-aee4-95632b147af1" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EffectOfExchangeRateOnCashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents_62edb812-dbd3-4ce6-8fef-3f3ad635beac" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EffectOfExchangeRateOnCashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_0e001e46-4975-4e59-8cb8-52042fa73a91" xlink:to="loc_us-gaap_EffectOfExchangeRateOnCashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents_62edb812-dbd3-4ce6-8fef-3f3ad635beac" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect_2ca982e0-45dd-4691-be0d-42cb1ea85973" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_0e001e46-4975-4e59-8cb8-52042fa73a91" xlink:to="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect_2ca982e0-45dd-4691-be0d-42cb1ea85973" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents_7efdcb85-bcc6-43f8-891e-f47c5df02761" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_0e001e46-4975-4e59-8cb8-52042fa73a91" xlink:to="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents_7efdcb85-bcc6-43f8-891e-f47c5df02761" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents_b558b76a-506d-4228-b9e0-650579eb9ffe" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxesPaidNet_0df5a90d-c368-4df2-87ea-91060b7c0e11" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncomeTaxesPaidNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_0e001e46-4975-4e59-8cb8-52042fa73a91" xlink:to="loc_us-gaap_IncomeTaxesPaidNet_0df5a90d-c368-4df2-87ea-91060b7c0e11" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SupplementalCashFlowInformationAbstract_9150d31a-1381-4599-bcef-7e035e4d750b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SupplementalCashFlowInformationAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_0e001e46-4975-4e59-8cb8-52042fa73a91" xlink:to="loc_us-gaap_SupplementalCashFlowInformationAbstract_9150d31a-1381-4599-bcef-7e035e4d750b" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherSignificantNoncashTransactionValueOfConsiderationGiven1_a32de314-9706-49fc-b31d-557f1d997e9b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OtherSignificantNoncashTransactionValueOfConsiderationGiven1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SupplementalCashFlowInformationAbstract_9150d31a-1381-4599-bcef-7e035e4d750b" xlink:to="loc_us-gaap_OtherSignificantNoncashTransactionValueOfConsiderationGiven1_a32de314-9706-49fc-b31d-557f1d997e9b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherSignificantNoncashTransactionValueOfConsiderationReceived1_9a7b1503-0091-45c8-a2bc-f24d8fb7782d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OtherSignificantNoncashTransactionValueOfConsiderationReceived1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SupplementalCashFlowInformationAbstract_9150d31a-1381-4599-bcef-7e035e4d750b" xlink:to="loc_us-gaap_OtherSignificantNoncashTransactionValueOfConsiderationReceived1_9a7b1503-0091-45c8-a2bc-f24d8fb7782d" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.centrusenergy.com/role/CONSOLIDATEDSTATEMENTSOFSTOCKHOLDERSDEFICIT" xlink:type="simple" xlink:href="leu-20240630.xsd#CONSOLIDATEDSTATEMENTSOFSTOCKHOLDERSDEFICIT"/>
  <link:definitionLink xlink:role="http://www.centrusenergy.com/role/CONSOLIDATEDSTATEMENTSOFSTOCKHOLDERSDEFICIT" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_b7f1cd74-a590-4eed-995b-d9e1adda6936" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_ea1e2b6f-f1f2-404f-a247-0510b464bebd" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_b7f1cd74-a590-4eed-995b-d9e1adda6936" xlink:to="loc_us-gaap_StatementTable_ea1e2b6f-f1f2-404f-a247-0510b464bebd" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementEquityComponentsAxis_c1fd67f7-8971-4445-9b25-228e4e77eb55" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementEquityComponentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_ea1e2b6f-f1f2-404f-a247-0510b464bebd" xlink:to="loc_us-gaap_StatementEquityComponentsAxis_c1fd67f7-8971-4445-9b25-228e4e77eb55" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_c1fd67f7-8971-4445-9b25-228e4e77eb55_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_c1fd67f7-8971-4445-9b25-228e4e77eb55" xlink:to="loc_us-gaap_EquityComponentDomain_c1fd67f7-8971-4445-9b25-228e4e77eb55_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_3ac93558-5a92-47e0-af9f-8fb4b7f47284" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_c1fd67f7-8971-4445-9b25-228e4e77eb55" xlink:to="loc_us-gaap_EquityComponentDomain_3ac93558-5a92-47e0-af9f-8fb4b7f47284" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockMember_9725dc70-e08b-4221-bd13-12d84f3c3954" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PreferredStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_3ac93558-5a92-47e0-af9f-8fb4b7f47284" xlink:to="loc_us-gaap_PreferredStockMember_9725dc70-e08b-4221-bd13-12d84f3c3954" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockMember_21c66029-bf9c-44c1-8111-2b9eb6b551ec" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommonStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_3ac93558-5a92-47e0-af9f-8fb4b7f47284" xlink:to="loc_us-gaap_CommonStockMember_21c66029-bf9c-44c1-8111-2b9eb6b551ec" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdditionalPaidInCapitalMember_a71f3b03-47ab-45cd-ac85-0080042e2aa5" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AdditionalPaidInCapitalMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_3ac93558-5a92-47e0-af9f-8fb4b7f47284" xlink:to="loc_us-gaap_AdditionalPaidInCapitalMember_a71f3b03-47ab-45cd-ac85-0080042e2aa5" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetainedEarningsMember_c2171567-0331-4a04-9bc6-66cc19132b52" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RetainedEarningsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_3ac93558-5a92-47e0-af9f-8fb4b7f47284" xlink:to="loc_us-gaap_RetainedEarningsMember_c2171567-0331-4a04-9bc6-66cc19132b52" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeMember_eeabe6d0-d5b4-467d-93ec-2cb8bc5870c5" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_3ac93558-5a92-47e0-af9f-8fb4b7f47284" xlink:to="loc_us-gaap_AccumulatedOtherComprehensiveIncomeMember_eeabe6d0-d5b4-467d-93ec-2cb8bc5870c5" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementClassOfStockAxis_a0ac43b1-8e23-4f09-ad96-e9370628f835" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementClassOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_ea1e2b6f-f1f2-404f-a247-0510b464bebd" xlink:to="loc_us-gaap_StatementClassOfStockAxis_a0ac43b1-8e23-4f09-ad96-e9370628f835" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_a0ac43b1-8e23-4f09-ad96-e9370628f835_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementClassOfStockAxis_a0ac43b1-8e23-4f09-ad96-e9370628f835" xlink:to="loc_us-gaap_ClassOfStockDomain_a0ac43b1-8e23-4f09-ad96-e9370628f835_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_cff041d4-2bd3-4493-b9e6-77db1278c60b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementClassOfStockAxis_a0ac43b1-8e23-4f09-ad96-e9370628f835" xlink:to="loc_us-gaap_ClassOfStockDomain_cff041d4-2bd3-4493-b9e6-77db1278c60b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonClassAMember_36b43349-9642-41f5-bbab-d65c42cfb909" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommonClassAMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_cff041d4-2bd3-4493-b9e6-77db1278c60b" xlink:to="loc_us-gaap_CommonClassAMember_36b43349-9642-41f5-bbab-d65c42cfb909" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonClassBMember_eb9cef39-3ce0-4544-afde-168d984b6b4a" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommonClassBMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_cff041d4-2bd3-4493-b9e6-77db1278c60b" xlink:to="loc_us-gaap_CommonClassBMember_eb9cef39-3ce0-4544-afde-168d984b6b4a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_64c4dc7d-3d57-413d-a832-2c860f4a8a7c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StockholdersEquity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_b7f1cd74-a590-4eed-995b-d9e1adda6936" xlink:to="loc_us-gaap_StockholdersEquity_64c4dc7d-3d57-413d-a832-2c860f4a8a7c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_b7888560-dc88-4468-8c5b-c4485f10bb9b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NetIncomeLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_b7f1cd74-a590-4eed-995b-d9e1adda6936" xlink:to="loc_us-gaap_NetIncomeLoss_b7888560-dc88-4468-8c5b-c4485f10bb9b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodValueStockOptionsExercised_2986e083-2f08-4baa-ab51-614d38ce173d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StockIssuedDuringPeriodValueStockOptionsExercised"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_b7f1cd74-a590-4eed-995b-d9e1adda6936" xlink:to="loc_us-gaap_StockIssuedDuringPeriodValueStockOptionsExercised_2986e083-2f08-4baa-ab51-614d38ce173d" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodValueNewIssues_f8e96321-78a8-42dc-94b7-d071f24e8b4f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StockIssuedDuringPeriodValueNewIssues"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_b7f1cd74-a590-4eed-995b-d9e1adda6936" xlink:to="loc_us-gaap_StockIssuedDuringPeriodValueNewIssues_f8e96321-78a8-42dc-94b7-d071f24e8b4f" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_ExchangeOfPreferredStockForCommonStockAndCommonStockWarrant_ed3596df-c3aa-4fd1-b158-691276328f81" xlink:href="leu-20240630.xsd#leu_ExchangeOfPreferredStockForCommonStockAndCommonStockWarrant"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_b7f1cd74-a590-4eed-995b-d9e1adda6936" xlink:to="loc_leu_ExchangeOfPreferredStockForCommonStockAndCommonStockWarrant_ed3596df-c3aa-4fd1-b158-691276328f81" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_ReclassificationOfStockBasedCompensationLiabilityToEquity_b4f138ab-cbb7-4f4f-9847-2ce16ecf2ec2" xlink:href="leu-20240630.xsd#leu_ReclassificationOfStockBasedCompensationLiabilityToEquity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_b7f1cd74-a590-4eed-995b-d9e1adda6936" xlink:to="loc_leu_ReclassificationOfStockBasedCompensationLiabilityToEquity_b4f138ab-cbb7-4f4f-9847-2ce16ecf2ec2" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdjustmentsRelatedToTaxWithholdingForShareBasedCompensation_06e3e00a-9df0-4902-86c6-b9519a42590c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AdjustmentsRelatedToTaxWithholdingForShareBasedCompensation"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_b7f1cd74-a590-4eed-995b-d9e1adda6936" xlink:to="loc_us-gaap_AdjustmentsRelatedToTaxWithholdingForShareBasedCompensation_06e3e00a-9df0-4902-86c6-b9519a42590c" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeDefinedBenefitPlansNetUnamortizedGainLossArisingDuringPeriodNetOfTax_e3e4d9bc-68db-494f-9d53-f74e94294bf6" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OtherComprehensiveIncomeDefinedBenefitPlansNetUnamortizedGainLossArisingDuringPeriodNetOfTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_b7f1cd74-a590-4eed-995b-d9e1adda6936" xlink:to="loc_us-gaap_OtherComprehensiveIncomeDefinedBenefitPlansNetUnamortizedGainLossArisingDuringPeriodNetOfTax_e3e4d9bc-68db-494f-9d53-f74e94294bf6" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodValueShareBasedCompensation_309f4624-7286-4fc4-aa16-4c46b2805760" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StockIssuedDuringPeriodValueShareBasedCompensation"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_b7f1cd74-a590-4eed-995b-d9e1adda6936" xlink:to="loc_us-gaap_StockIssuedDuringPeriodValueShareBasedCompensation_309f4624-7286-4fc4-aa16-4c46b2805760" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_76a4e512-5b2b-4c66-a90e-d48b8247c332" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StockholdersEquity"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.centrusenergy.com/role/PensionandPostretirementHealthandLifeBenefitsTables" xlink:type="simple" xlink:href="leu-20240630.xsd#PensionandPostretirementHealthandLifeBenefitsTables"/>
  <link:definitionLink xlink:role="http://www.centrusenergy.com/role/PensionandPostretirementHealthandLifeBenefitsTables" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_d9ca0c52-f20a-4f59-beb9-6359dc910cdf" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DefinedBenefitPlanDisclosureLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable_0be5bc31-ff3e-4f4c-834d-1e6781f20758" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_d9ca0c52-f20a-4f59-beb9-6359dc910cdf" xlink:to="loc_us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable_0be5bc31-ff3e-4f4c-834d-1e6781f20758" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetirementPlanTypeAxis_4ece7c63-aaef-4025-a9f8-4a65d82dd737" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RetirementPlanTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable_0be5bc31-ff3e-4f4c-834d-1e6781f20758" xlink:to="loc_us-gaap_RetirementPlanTypeAxis_4ece7c63-aaef-4025-a9f8-4a65d82dd737" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetirementPlanTypeDomain_4ece7c63-aaef-4025-a9f8-4a65d82dd737_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RetirementPlanTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_RetirementPlanTypeAxis_4ece7c63-aaef-4025-a9f8-4a65d82dd737" xlink:to="loc_us-gaap_RetirementPlanTypeDomain_4ece7c63-aaef-4025-a9f8-4a65d82dd737_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetirementPlanTypeDomain_85501ec0-8352-4770-93f3-82ba85906a20" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RetirementPlanTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_RetirementPlanTypeAxis_4ece7c63-aaef-4025-a9f8-4a65d82dd737" xlink:to="loc_us-gaap_RetirementPlanTypeDomain_85501ec0-8352-4770-93f3-82ba85906a20" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PensionPlansDefinedBenefitMember_935d0f26-08ca-439d-9fac-e428dea131ad" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PensionPlansDefinedBenefitMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RetirementPlanTypeDomain_85501ec0-8352-4770-93f3-82ba85906a20" xlink:to="loc_us-gaap_PensionPlansDefinedBenefitMember_935d0f26-08ca-439d-9fac-e428dea131ad" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherPostretirementBenefitPlansDefinedBenefitMember_e57a5d5e-262b-4175-9f64-52de222b725d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OtherPostretirementBenefitPlansDefinedBenefitMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RetirementPlanTypeDomain_85501ec0-8352-4770-93f3-82ba85906a20" xlink:to="loc_us-gaap_OtherPostretirementBenefitPlansDefinedBenefitMember_e57a5d5e-262b-4175-9f64-52de222b725d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfNetBenefitCostsTableTextBlock_0600ce43-034c-4512-9cad-c58786f015bf" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ScheduleOfNetBenefitCostsTableTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_d9ca0c52-f20a-4f59-beb9-6359dc910cdf" xlink:to="loc_us-gaap_ScheduleOfNetBenefitCostsTableTextBlock_0600ce43-034c-4512-9cad-c58786f015bf" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.centrusenergy.com/role/RevenueandContractswithCustomersRevenuefromExternalCustomersbyGeographicAreasDetails" xlink:type="simple" xlink:href="leu-20240630.xsd#RevenueandContractswithCustomersRevenuefromExternalCustomersbyGeographicAreasDetails"/>
  <link:definitionLink xlink:role="http://www.centrusenergy.com/role/RevenueandContractswithCustomersRevenuefromExternalCustomersbyGeographicAreasDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_f4c33d62-06b7-4d2d-9c7b-16ab83e52f82" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_7fbca130-33eb-473d-aca7-70756d7fe871" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_f4c33d62-06b7-4d2d-9c7b-16ab83e52f82" xlink:to="loc_us-gaap_StatementTable_7fbca130-33eb-473d-aca7-70756d7fe871" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductOrServiceAxis_dba11185-6e6e-4a7b-9c2f-c4cf3c4b9185" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_ProductOrServiceAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_7fbca130-33eb-473d-aca7-70756d7fe871" xlink:to="loc_srt_ProductOrServiceAxis_dba11185-6e6e-4a7b-9c2f-c4cf3c4b9185" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_dba11185-6e6e-4a7b-9c2f-c4cf3c4b9185_default" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_ProductOrServiceAxis_dba11185-6e6e-4a7b-9c2f-c4cf3c4b9185" xlink:to="loc_srt_ProductsAndServicesDomain_dba11185-6e6e-4a7b-9c2f-c4cf3c4b9185_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_d04ec0b1-f13b-454a-9c12-d87b3fc4be85" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_ProductOrServiceAxis_dba11185-6e6e-4a7b-9c2f-c4cf3c4b9185" xlink:to="loc_srt_ProductsAndServicesDomain_d04ec0b1-f13b-454a-9c12-d87b3fc4be85" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProductMember_5a4a0804-063d-4533-8c69-b7024fe0b6b9" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ProductMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_d04ec0b1-f13b-454a-9c12-d87b3fc4be85" xlink:to="loc_us-gaap_ProductMember_5a4a0804-063d-4533-8c69-b7024fe0b6b9" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_StatementGeographicalAxis_242ad790-50b6-4125-97a6-e99712ceccab" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_StatementGeographicalAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_7fbca130-33eb-473d-aca7-70756d7fe871" xlink:to="loc_srt_StatementGeographicalAxis_242ad790-50b6-4125-97a6-e99712ceccab" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_242ad790-50b6-4125-97a6-e99712ceccab_default" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_SegmentGeographicalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_StatementGeographicalAxis_242ad790-50b6-4125-97a6-e99712ceccab" xlink:to="loc_srt_SegmentGeographicalDomain_242ad790-50b6-4125-97a6-e99712ceccab_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_894150bb-8348-4434-aea7-f6a8910d0875" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_SegmentGeographicalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_StatementGeographicalAxis_242ad790-50b6-4125-97a6-e99712ceccab" xlink:to="loc_srt_SegmentGeographicalDomain_894150bb-8348-4434-aea7-f6a8910d0875" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_country_US_56564443-3bd5-49a8-a4fe-09d8672416bd" xlink:href="https://xbrl.sec.gov/country/2024/country-2024.xsd#country_US"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_894150bb-8348-4434-aea7-f6a8910d0875" xlink:to="loc_country_US_56564443-3bd5-49a8-a4fe-09d8672416bd" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_OtherForeignMember_ec4e9710-5305-428b-b884-cc4c0a001f57" xlink:href="leu-20240630.xsd#leu_OtherForeignMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_894150bb-8348-4434-aea7-f6a8910d0875" xlink:to="loc_leu_OtherForeignMember_ec4e9710-5305-428b-b884-cc4c0a001f57" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_a6c54553-12ef-43e3-9b8f-aa16a7bf2b4a" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_f4c33d62-06b7-4d2d-9c7b-16ab83e52f82" xlink:to="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_a6c54553-12ef-43e3-9b8f-aa16a7bf2b4a" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.centrusenergy.com/role/RevenueandContractswithCustomersContractwithCustomerAssetandLiabilityDetails" xlink:type="simple" xlink:href="leu-20240630.xsd#RevenueandContractswithCustomersContractwithCustomerAssetandLiabilityDetails"/>
  <link:definitionLink xlink:role="http://www.centrusenergy.com/role/RevenueandContractswithCustomersContractwithCustomerAssetandLiabilityDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_006faf8e-a48c-4654-9cee-9363ad04dfce" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_cdb5c398-6124-4fd3-bc41-29f09de7a8fa" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_006faf8e-a48c-4654-9cee-9363ad04dfce" xlink:to="loc_us-gaap_StatementTable_cdb5c398-6124-4fd3-bc41-29f09de7a8fa" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountsNotesLoansAndFinancingReceivablesByLegalEntityOfCounterpartyTypeAxis_9db41f95-db33-478d-9dec-4ef803f69d42" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccountsNotesLoansAndFinancingReceivablesByLegalEntityOfCounterpartyTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_cdb5c398-6124-4fd3-bc41-29f09de7a8fa" xlink:to="loc_us-gaap_AccountsNotesLoansAndFinancingReceivablesByLegalEntityOfCounterpartyTypeAxis_9db41f95-db33-478d-9dec-4ef803f69d42" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LegalEntityTypeOfCounterpartyDomain_9db41f95-db33-478d-9dec-4ef803f69d42_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LegalEntityTypeOfCounterpartyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AccountsNotesLoansAndFinancingReceivablesByLegalEntityOfCounterpartyTypeAxis_9db41f95-db33-478d-9dec-4ef803f69d42" xlink:to="loc_us-gaap_LegalEntityTypeOfCounterpartyDomain_9db41f95-db33-478d-9dec-4ef803f69d42_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LegalEntityTypeOfCounterpartyDomain_5c025830-c6fb-4e60-9a07-e06049fc6fe9" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LegalEntityTypeOfCounterpartyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AccountsNotesLoansAndFinancingReceivablesByLegalEntityOfCounterpartyTypeAxis_9db41f95-db33-478d-9dec-4ef803f69d42" xlink:to="loc_us-gaap_LegalEntityTypeOfCounterpartyDomain_5c025830-c6fb-4e60-9a07-e06049fc6fe9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GovernmentMember_84bea61d-21d7-4377-86a8-b8798f3ad8d9" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_GovernmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LegalEntityTypeOfCounterpartyDomain_5c025830-c6fb-4e60-9a07-e06049fc6fe9" xlink:to="loc_us-gaap_GovernmentMember_84bea61d-21d7-4377-86a8-b8798f3ad8d9" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProvisionForLossOnContracts_2a9abb32-faff-4e6f-8b1a-67ee17c76d91" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ProvisionForLossOnContracts"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_006faf8e-a48c-4654-9cee-9363ad04dfce" xlink:to="loc_us-gaap_ProvisionForLossOnContracts_2a9abb32-faff-4e6f-8b1a-67ee17c76d91" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_ProvisionForLossOnContractChange_c40e8a26-f4a5-440c-83aa-421c15681f5b" xlink:href="leu-20240630.xsd#leu_ProvisionForLossOnContractChange"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_006faf8e-a48c-4654-9cee-9363ad04dfce" xlink:to="loc_leu_ProvisionForLossOnContractChange_c40e8a26-f4a5-440c-83aa-421c15681f5b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredRevenue_ef92c9b5-c8cd-41ac-a7b1-ffa6e712a232" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DeferredRevenue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_006faf8e-a48c-4654-9cee-9363ad04dfce" xlink:to="loc_us-gaap_DeferredRevenue_ef92c9b5-c8cd-41ac-a7b1-ffa6e712a232" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInDeferredRevenue_db367371-a3cf-42dd-ac70-ccd6efa9aedf" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncreaseDecreaseInDeferredRevenue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_006faf8e-a48c-4654-9cee-9363ad04dfce" xlink:to="loc_us-gaap_IncreaseDecreaseInDeferredRevenue_db367371-a3cf-42dd-ac70-ccd6efa9aedf" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_ContractWithCustomerAdvancesLiabilityCurrent_43a5b71a-1491-4434-acf4-d07b720ffcef" xlink:href="leu-20240630.xsd#leu_ContractWithCustomerAdvancesLiabilityCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_006faf8e-a48c-4654-9cee-9363ad04dfce" xlink:to="loc_leu_ContractWithCustomerAdvancesLiabilityCurrent_43a5b71a-1491-4434-acf4-d07b720ffcef" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_AdvancesFromCustomersChange_3485d47a-d286-4155-9feb-1f004d918a75" xlink:href="leu-20240630.xsd#leu_AdvancesFromCustomersChange"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_006faf8e-a48c-4654-9cee-9363ad04dfce" xlink:to="loc_leu_AdvancesFromCustomersChange_3485d47a-d286-4155-9feb-1f004d918a75" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredRevenueNoncurrent_af8f1d10-d47b-4c7f-814e-e11bc3db2793" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DeferredRevenueNoncurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_006faf8e-a48c-4654-9cee-9363ad04dfce" xlink:to="loc_us-gaap_DeferredRevenueNoncurrent_af8f1d10-d47b-4c7f-814e-e11bc3db2793" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_IncreasedecreaseinAdvancesfromcustomersnoncurrent_9bc3788a-9512-42c2-8be3-b83d55e86d34" xlink:href="leu-20240630.xsd#leu_IncreasedecreaseinAdvancesfromcustomersnoncurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_006faf8e-a48c-4654-9cee-9363ad04dfce" xlink:to="loc_leu_IncreasedecreaseinAdvancesfromcustomersnoncurrent_9bc3788a-9512-42c2-8be3-b83d55e86d34" xlink:type="arc" order="7"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.centrusenergy.com/role/RevenueandContractswithCustomersNarrativeDetails" xlink:type="simple" xlink:href="leu-20240630.xsd#RevenueandContractswithCustomersNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.centrusenergy.com/role/RevenueandContractswithCustomersNarrativeDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_d9d43d42-c8eb-45bf-9279-106c7b10f425" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccountsNotesAndLoansReceivableLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfAccountsNotesLoansAndFinancingReceivableTable_7c179cd1-29d3-4c50-bd22-d4d80793286f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ScheduleOfAccountsNotesLoansAndFinancingReceivableTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_d9d43d42-c8eb-45bf-9279-106c7b10f425" xlink:to="loc_us-gaap_ScheduleOfAccountsNotesLoansAndFinancingReceivableTable_7c179cd1-29d3-4c50-bd22-d4d80793286f" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_7c540b9a-5cc1-48bf-be15-935afb70d668" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfAccountsNotesLoansAndFinancingReceivableTable_7c179cd1-29d3-4c50-bd22-d4d80793286f" xlink:to="loc_srt_RangeAxis_7c540b9a-5cc1-48bf-be15-935afb70d668" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_7c540b9a-5cc1-48bf-be15-935afb70d668_default" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_7c540b9a-5cc1-48bf-be15-935afb70d668" xlink:to="loc_srt_RangeMember_7c540b9a-5cc1-48bf-be15-935afb70d668_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_04fee9dd-31d2-4207-89b1-85bcace417d5" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_7c540b9a-5cc1-48bf-be15-935afb70d668" xlink:to="loc_srt_RangeMember_04fee9dd-31d2-4207-89b1-85bcace417d5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_2caa4610-2c42-4d6d-864c-80453cdda50f" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_04fee9dd-31d2-4207-89b1-85bcace417d5" xlink:to="loc_srt_MaximumMember_2caa4610-2c42-4d6d-864c-80453cdda50f" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountsNotesLoansAndFinancingReceivablesByLegalEntityOfCounterpartyTypeAxis_3fccc754-1f42-4c79-8c76-e397be01affa" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccountsNotesLoansAndFinancingReceivablesByLegalEntityOfCounterpartyTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfAccountsNotesLoansAndFinancingReceivableTable_7c179cd1-29d3-4c50-bd22-d4d80793286f" xlink:to="loc_us-gaap_AccountsNotesLoansAndFinancingReceivablesByLegalEntityOfCounterpartyTypeAxis_3fccc754-1f42-4c79-8c76-e397be01affa" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LegalEntityTypeOfCounterpartyDomain_3fccc754-1f42-4c79-8c76-e397be01affa_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LegalEntityTypeOfCounterpartyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AccountsNotesLoansAndFinancingReceivablesByLegalEntityOfCounterpartyTypeAxis_3fccc754-1f42-4c79-8c76-e397be01affa" xlink:to="loc_us-gaap_LegalEntityTypeOfCounterpartyDomain_3fccc754-1f42-4c79-8c76-e397be01affa_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LegalEntityTypeOfCounterpartyDomain_19f70974-dc75-41c4-8d55-bb688e54c266" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LegalEntityTypeOfCounterpartyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AccountsNotesLoansAndFinancingReceivablesByLegalEntityOfCounterpartyTypeAxis_3fccc754-1f42-4c79-8c76-e397be01affa" xlink:to="loc_us-gaap_LegalEntityTypeOfCounterpartyDomain_19f70974-dc75-41c4-8d55-bb688e54c266" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GovernmentMember_8e4cbc7c-589f-4ec2-a41e-c7c4b2afcd8d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_GovernmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LegalEntityTypeOfCounterpartyDomain_19f70974-dc75-41c4-8d55-bb688e54c266" xlink:to="loc_us-gaap_GovernmentMember_8e4cbc7c-589f-4ec2-a41e-c7c4b2afcd8d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetirementPlanTypeAxis_1139b677-8ea1-41b2-bbdf-ac9be2622a5c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RetirementPlanTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfAccountsNotesLoansAndFinancingReceivableTable_7c179cd1-29d3-4c50-bd22-d4d80793286f" xlink:to="loc_us-gaap_RetirementPlanTypeAxis_1139b677-8ea1-41b2-bbdf-ac9be2622a5c" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetirementPlanTypeDomain_1139b677-8ea1-41b2-bbdf-ac9be2622a5c_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RetirementPlanTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_RetirementPlanTypeAxis_1139b677-8ea1-41b2-bbdf-ac9be2622a5c" xlink:to="loc_us-gaap_RetirementPlanTypeDomain_1139b677-8ea1-41b2-bbdf-ac9be2622a5c_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetirementPlanTypeDomain_1aabb545-1e8d-4321-a0f1-1b825beade06" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RetirementPlanTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_RetirementPlanTypeAxis_1139b677-8ea1-41b2-bbdf-ac9be2622a5c" xlink:to="loc_us-gaap_RetirementPlanTypeDomain_1aabb545-1e8d-4321-a0f1-1b825beade06" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherPostretirementBenefitPlansDefinedBenefitMember_720e2680-074a-42ef-b375-76946f5999c0" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OtherPostretirementBenefitPlansDefinedBenefitMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RetirementPlanTypeDomain_1aabb545-1e8d-4321-a0f1-1b825beade06" xlink:to="loc_us-gaap_OtherPostretirementBenefitPlansDefinedBenefitMember_720e2680-074a-42ef-b375-76946f5999c0" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PensionPlansDefinedBenefitMember_f4cf6b25-1ffb-4a44-a3bb-6039e178ce12" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PensionPlansDefinedBenefitMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RetirementPlanTypeDomain_1aabb545-1e8d-4321-a0f1-1b825beade06" xlink:to="loc_us-gaap_PensionPlansDefinedBenefitMember_f4cf6b25-1ffb-4a44-a3bb-6039e178ce12" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ContractWithCustomerSalesChannelAxis_4e7702ee-126b-4b1b-b075-d1222c555569" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ContractWithCustomerSalesChannelAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfAccountsNotesLoansAndFinancingReceivableTable_7c179cd1-29d3-4c50-bd22-d4d80793286f" xlink:to="loc_us-gaap_ContractWithCustomerSalesChannelAxis_4e7702ee-126b-4b1b-b075-d1222c555569" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ContractWithCustomerSalesChannelDomain_4e7702ee-126b-4b1b-b075-d1222c555569_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ContractWithCustomerSalesChannelDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_ContractWithCustomerSalesChannelAxis_4e7702ee-126b-4b1b-b075-d1222c555569" xlink:to="loc_us-gaap_ContractWithCustomerSalesChannelDomain_4e7702ee-126b-4b1b-b075-d1222c555569_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ContractWithCustomerSalesChannelDomain_141e5620-54a8-42cc-8c23-f05e3cd8d6bc" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ContractWithCustomerSalesChannelDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_ContractWithCustomerSalesChannelAxis_4e7702ee-126b-4b1b-b075-d1222c555569" xlink:to="loc_us-gaap_ContractWithCustomerSalesChannelDomain_141e5620-54a8-42cc-8c23-f05e3cd8d6bc" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ContractWithCustomerDurationAxis_b605115d-e9fb-4ca0-86a2-20e6af134ab8" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ContractWithCustomerDurationAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfAccountsNotesLoansAndFinancingReceivableTable_7c179cd1-29d3-4c50-bd22-d4d80793286f" xlink:to="loc_us-gaap_ContractWithCustomerDurationAxis_b605115d-e9fb-4ca0-86a2-20e6af134ab8" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ContractWithCustomerDurationDomain_b605115d-e9fb-4ca0-86a2-20e6af134ab8_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ContractWithCustomerDurationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_ContractWithCustomerDurationAxis_b605115d-e9fb-4ca0-86a2-20e6af134ab8" xlink:to="loc_us-gaap_ContractWithCustomerDurationDomain_b605115d-e9fb-4ca0-86a2-20e6af134ab8_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ContractWithCustomerDurationDomain_0d108972-e9f9-46d1-9767-fd42653adf1a" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ContractWithCustomerDurationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_ContractWithCustomerDurationAxis_b605115d-e9fb-4ca0-86a2-20e6af134ab8" xlink:to="loc_us-gaap_ContractWithCustomerDurationDomain_0d108972-e9f9-46d1-9767-fd42653adf1a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_HALEUDemoContractMember_3ccaa0f1-34fa-4ba9-b8fc-e38ef1960d9a" xlink:href="leu-20240630.xsd#leu_HALEUDemoContractMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ContractWithCustomerDurationDomain_0d108972-e9f9-46d1-9767-fd42653adf1a" xlink:to="loc_leu_HALEUDemoContractMember_3ccaa0f1-34fa-4ba9-b8fc-e38ef1960d9a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_HALEUOpsContractMember_9a9aaed6-055a-4002-85fc-d0ea6cac40fc" xlink:href="leu-20240630.xsd#leu_HALEUOpsContractMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ContractWithCustomerDurationDomain_0d108972-e9f9-46d1-9767-fd42653adf1a" xlink:to="loc_leu_HALEUOpsContractMember_9a9aaed6-055a-4002-85fc-d0ea6cac40fc" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_ContractPhaseAxis_8b825922-6153-4910-8222-df1daa33a215" xlink:href="leu-20240630.xsd#leu_ContractPhaseAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfAccountsNotesLoansAndFinancingReceivableTable_7c179cd1-29d3-4c50-bd22-d4d80793286f" xlink:to="loc_leu_ContractPhaseAxis_8b825922-6153-4910-8222-df1daa33a215" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_ContractPhaseDomain_8b825922-6153-4910-8222-df1daa33a215_default" xlink:href="leu-20240630.xsd#leu_ContractPhaseDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_leu_ContractPhaseAxis_8b825922-6153-4910-8222-df1daa33a215" xlink:to="loc_leu_ContractPhaseDomain_8b825922-6153-4910-8222-df1daa33a215_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_ContractPhaseDomain_7a14b003-780d-4b5d-93ef-0b250c56e988" xlink:href="leu-20240630.xsd#leu_ContractPhaseDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_leu_ContractPhaseAxis_8b825922-6153-4910-8222-df1daa33a215" xlink:to="loc_leu_ContractPhaseDomain_7a14b003-780d-4b5d-93ef-0b250c56e988" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_Phase1Member_d463d04f-fab5-4b6f-ba57-737e698aaf74" xlink:href="leu-20240630.xsd#leu_Phase1Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_leu_ContractPhaseDomain_7a14b003-780d-4b5d-93ef-0b250c56e988" xlink:to="loc_leu_Phase1Member_d463d04f-fab5-4b6f-ba57-737e698aaf74" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_Phase2Member_8dbe426a-d733-4997-a51e-f51ec269dc6f" xlink:href="leu-20240630.xsd#leu_Phase2Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_leu_ContractPhaseDomain_7a14b003-780d-4b5d-93ef-0b250c56e988" xlink:to="loc_leu_Phase2Member_8dbe426a-d733-4997-a51e-f51ec269dc6f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventTypeAxis_6152f94a-2c85-4619-b051-30f62048472b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SubsequentEventTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfAccountsNotesLoansAndFinancingReceivableTable_7c179cd1-29d3-4c50-bd22-d4d80793286f" xlink:to="loc_us-gaap_SubsequentEventTypeAxis_6152f94a-2c85-4619-b051-30f62048472b" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventTypeDomain_6152f94a-2c85-4619-b051-30f62048472b_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SubsequentEventTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_SubsequentEventTypeAxis_6152f94a-2c85-4619-b051-30f62048472b" xlink:to="loc_us-gaap_SubsequentEventTypeDomain_6152f94a-2c85-4619-b051-30f62048472b_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventTypeDomain_43e4710f-0856-483c-8afd-114665838b2b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SubsequentEventTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_SubsequentEventTypeAxis_6152f94a-2c85-4619-b051-30f62048472b" xlink:to="loc_us-gaap_SubsequentEventTypeDomain_43e4710f-0856-483c-8afd-114665838b2b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventMember_266ab5c4-2434-464f-ae81-130d60d86b57" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SubsequentEventMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsequentEventTypeDomain_43e4710f-0856-483c-8afd-114665838b2b" xlink:to="loc_us-gaap_SubsequentEventMember_266ab5c4-2434-464f-ae81-130d60d86b57" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementBusinessSegmentsAxis_ab032f4d-f767-4829-942d-78cb4e734f16" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementBusinessSegmentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfAccountsNotesLoansAndFinancingReceivableTable_7c179cd1-29d3-4c50-bd22-d4d80793286f" xlink:to="loc_us-gaap_StatementBusinessSegmentsAxis_ab032f4d-f767-4829-942d-78cb4e734f16" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentDomain_ab032f4d-f767-4829-942d-78cb4e734f16_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementBusinessSegmentsAxis_ab032f4d-f767-4829-942d-78cb4e734f16" xlink:to="loc_us-gaap_SegmentDomain_ab032f4d-f767-4829-942d-78cb4e734f16_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentDomain_80546ff3-df65-4a1c-b188-44797458d749" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementBusinessSegmentsAxis_ab032f4d-f767-4829-942d-78cb4e734f16" xlink:to="loc_us-gaap_SegmentDomain_80546ff3-df65-4a1c-b188-44797458d749" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_LEUSegmentMember_74fd4827-c9c0-448d-b242-8f0c81844b16" xlink:href="leu-20240630.xsd#leu_LEUSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_80546ff3-df65-4a1c-b188-44797458d749" xlink:to="loc_leu_LEUSegmentMember_74fd4827-c9c0-448d-b242-8f0c81844b16" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_SWUSegmentMember_38a153f8-4f65-454b-bc19-1fe2b2edf837" xlink:href="leu-20240630.xsd#leu_SWUSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_80546ff3-df65-4a1c-b188-44797458d749" xlink:to="loc_leu_SWUSegmentMember_38a153f8-4f65-454b-bc19-1fe2b2edf837" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_TechnicalSolutionsSegmentMember_6f625ab0-10ad-417a-aae1-15a9483af9aa" xlink:href="leu-20240630.xsd#leu_TechnicalSolutionsSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_80546ff3-df65-4a1c-b188-44797458d749" xlink:to="loc_leu_TechnicalSolutionsSegmentMember_6f625ab0-10ad-417a-aae1-15a9483af9aa" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_GovernmentCostSharePercentage_a8c765ca-68e2-413c-a643-ce2bfff87157" xlink:href="leu-20240630.xsd#leu_GovernmentCostSharePercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_d9d43d42-c8eb-45bf-9279-106c7b10f425" xlink:to="loc_leu_GovernmentCostSharePercentage_a8c765ca-68e2-413c-a643-ce2bfff87157" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_Governmentcostshareportion_69176f64-7117-4147-81a9-e54e7443c749" xlink:href="leu-20240630.xsd#leu_Governmentcostshareportion"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_d9d43d42-c8eb-45bf-9279-106c7b10f425" xlink:to="loc_leu_Governmentcostshareportion_69176f64-7117-4147-81a9-e54e7443c749" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_ProvisionForLossOnContractsUtilized_a1e7b68a-c74d-40e5-a794-2ac4f8ccbb9b" xlink:href="leu-20240630.xsd#leu_ProvisionForLossOnContractsUtilized"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_d9d43d42-c8eb-45bf-9279-106c7b10f425" xlink:to="loc_leu_ProvisionForLossOnContractsUtilized_a1e7b68a-c74d-40e5-a794-2ac4f8ccbb9b" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProvisionForLossOnContracts_c08f7009-9aaa-486c-a2c2-e754be329cff" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ProvisionForLossOnContracts"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_d9d43d42-c8eb-45bf-9279-106c7b10f425" xlink:to="loc_us-gaap_ProvisionForLossOnContracts_c08f7009-9aaa-486c-a2c2-e754be329cff" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromCustomers_1f675964-44c9-4a0e-8cf0-7df9e4bf14ad" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ProceedsFromCustomers"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_d9d43d42-c8eb-45bf-9279-106c7b10f425" xlink:to="loc_us-gaap_ProceedsFromCustomers_1f675964-44c9-4a0e-8cf0-7df9e4bf14ad" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredRevenueRevenueRecognized1_c9e7e9f8-48ff-417d-87fa-910cb1c7101d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DeferredRevenueRevenueRecognized1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_d9d43d42-c8eb-45bf-9279-106c7b10f425" xlink:to="loc_us-gaap_DeferredRevenueRevenueRecognized1_c9e7e9f8-48ff-417d-87fa-910cb1c7101d" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueRemainingPerformanceObligation_54d94139-e60b-4680-a88e-14561771c5f3" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RevenueRemainingPerformanceObligation"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_d9d43d42-c8eb-45bf-9279-106c7b10f425" xlink:to="loc_us-gaap_RevenueRemainingPerformanceObligation_54d94139-e60b-4680-a88e-14561771c5f3" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_DeferredRevenueAndAdvancesFromCustomersCurrentAndNoncurrent_ee623971-73e7-47a4-9bff-d4287b6b8506" xlink:href="leu-20240630.xsd#leu_DeferredRevenueAndAdvancesFromCustomersCurrentAndNoncurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_d9d43d42-c8eb-45bf-9279-106c7b10f425" xlink:to="loc_leu_DeferredRevenueAndAdvancesFromCustomersCurrentAndNoncurrent_ee623971-73e7-47a4-9bff-d4287b6b8506" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_CostObligatedToDOE_bf9b164c-e4f4-4db4-8842-b7daa193fdab" xlink:href="leu-20240630.xsd#leu_CostObligatedToDOE"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_d9d43d42-c8eb-45bf-9279-106c7b10f425" xlink:to="loc_leu_CostObligatedToDOE_bf9b164c-e4f4-4db4-8842-b7daa193fdab" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_TotalAmountProgramCost_bb195260-1f3c-4c04-a173-9ada14f7cc5e" xlink:href="leu-20240630.xsd#leu_TotalAmountProgramCost"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_d9d43d42-c8eb-45bf-9279-106c7b10f425" xlink:to="loc_leu_TotalAmountProgramCost_bb195260-1f3c-4c04-a173-9ada14f7cc5e" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_ContractValue_90a38a66-3e4c-458f-8880-71cf681e715c" xlink:href="leu-20240630.xsd#leu_ContractValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_d9d43d42-c8eb-45bf-9279-106c7b10f425" xlink:to="loc_leu_ContractValue_90a38a66-3e4c-458f-8880-71cf681e715c" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_CompanyCostSharePortion_5dd0f765-9059-4bff-a476-2c08825351fd" xlink:href="leu-20240630.xsd#leu_CompanyCostSharePortion"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_d9d43d42-c8eb-45bf-9279-106c7b10f425" xlink:to="loc_leu_CompanyCostSharePortion_5dd0f765-9059-4bff-a476-2c08825351fd" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_NewAmountProgramCost_3376eea5-b27d-4876-b163-687a0eebe5c8" xlink:href="leu-20240630.xsd#leu_NewAmountProgramCost"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_d9d43d42-c8eb-45bf-9279-106c7b10f425" xlink:to="loc_leu_NewAmountProgramCost_3376eea5-b27d-4876-b163-687a0eebe5c8" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_AdditionalCostsObligatedToDOE_d0ceaef3-846a-4763-ad9c-fb6e34cb2910" xlink:href="leu-20240630.xsd#leu_AdditionalCostsObligatedToDOE"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_d9d43d42-c8eb-45bf-9279-106c7b10f425" xlink:to="loc_leu_AdditionalCostsObligatedToDOE_d0ceaef3-846a-4763-ad9c-fb6e34cb2910" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ForeignCurrencyTransactionGainLossUnrealized_89592ee6-e3fd-439c-b702-42bfa73f8372" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ForeignCurrencyTransactionGainLossUnrealized"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_d9d43d42-c8eb-45bf-9279-106c7b10f425" xlink:to="loc_us-gaap_ForeignCurrencyTransactionGainLossUnrealized_89592ee6-e3fd-439c-b702-42bfa73f8372" xlink:type="arc" order="14"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_AccruedLoss_33b4efc6-ba7e-4d81-9443-b234f095a5a8" xlink:href="leu-20240630.xsd#leu_AccruedLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_d9d43d42-c8eb-45bf-9279-106c7b10f425" xlink:to="loc_leu_AccruedLoss_33b4efc6-ba7e-4d81-9443-b234f095a5a8" xlink:type="arc" order="15"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.centrusenergy.com/role/CashCashEquivalentsandRestrictedCashScheduleofRestrictedCashDetails" xlink:type="simple" xlink:href="leu-20240630.xsd#CashCashEquivalentsandRestrictedCashScheduleofRestrictedCashDetails"/>
  <link:definitionLink xlink:role="http://www.centrusenergy.com/role/CashCashEquivalentsandRestrictedCashScheduleofRestrictedCashDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedCashAndCashEquivalentsItemsLineItems_64fa265c-9fae-4481-918e-4cd0432ea77d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RestrictedCashAndCashEquivalentsItemsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfRestrictedCashAndCashEquivalentsTable_53c0a675-a624-4a6e-a15b-5e17777138a4" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ScheduleOfRestrictedCashAndCashEquivalentsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_RestrictedCashAndCashEquivalentsItemsLineItems_64fa265c-9fae-4481-918e-4cd0432ea77d" xlink:to="loc_us-gaap_ScheduleOfRestrictedCashAndCashEquivalentsTable_53c0a675-a624-4a6e-a15b-5e17777138a4" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsAxis_db28c068-b2ce-465e-92b3-cea37c198a91" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRestrictedCashAndCashEquivalentsTable_53c0a675-a624-4a6e-a15b-5e17777138a4" xlink:to="loc_us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsAxis_db28c068-b2ce-465e-92b3-cea37c198a91" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsMember_db28c068-b2ce-465e-92b3-cea37c198a91_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsAxis_db28c068-b2ce-465e-92b3-cea37c198a91" xlink:to="loc_us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsMember_db28c068-b2ce-465e-92b3-cea37c198a91_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsMember_8a496075-e39c-4242-851c-e9fdf9bdc098" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsAxis_db28c068-b2ce-465e-92b3-cea37c198a91" xlink:to="loc_us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsMember_8a496075-e39c-4242-851c-e9fdf9bdc098" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_CollateralForInventoryLoanMember_4c6fe3fb-88b2-4abc-8335-8d51a1e372c6" xlink:href="leu-20240630.xsd#leu_CollateralForInventoryLoanMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsMember_8a496075-e39c-4242-851c-e9fdf9bdc098" xlink:to="loc_leu_CollateralForInventoryLoanMember_4c6fe3fb-88b2-4abc-8335-8d51a1e372c6" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_WorkerscompensationfinancialassuranceMember_a6bb11ad-afb1-4080-a825-12b8cb92a8f8" xlink:href="leu-20240630.xsd#leu_WorkerscompensationfinancialassuranceMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsMember_8a496075-e39c-4242-851c-e9fdf9bdc098" xlink:to="loc_leu_WorkerscompensationfinancialassuranceMember_a6bb11ad-afb1-4080-a825-12b8cb92a8f8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_OtherfinancialassuranceMember_0e91203e-a047-48b3-8c1f-a592c7f38343" xlink:href="leu-20240630.xsd#leu_OtherfinancialassuranceMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsMember_8a496075-e39c-4242-851c-e9fdf9bdc098" xlink:to="loc_leu_OtherfinancialassuranceMember_0e91203e-a047-48b3-8c1f-a592c7f38343" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedCashAndCashEquivalentsAtCarryingValue_226cf0e5-a980-4976-96a2-2e39c36ca204" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RestrictedCashAndCashEquivalentsAtCarryingValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RestrictedCashAndCashEquivalentsItemsLineItems_64fa265c-9fae-4481-918e-4cd0432ea77d" xlink:to="loc_us-gaap_RestrictedCashAndCashEquivalentsAtCarryingValue_226cf0e5-a980-4976-96a2-2e39c36ca204" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashHeldInForeignCurrency_e00c60c2-ac20-47ba-ae2a-fac212554fb8" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CashHeldInForeignCurrency"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RestrictedCashAndCashEquivalentsItemsLineItems_64fa265c-9fae-4481-918e-4cd0432ea77d" xlink:to="loc_us-gaap_CashHeldInForeignCurrency_e00c60c2-ac20-47ba-ae2a-fac212554fb8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedCashAndCashEquivalentsNoncurrent_45fe1207-33a0-4196-be8b-f9d78f080906" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RestrictedCashAndCashEquivalentsNoncurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RestrictedCashAndCashEquivalentsItemsLineItems_64fa265c-9fae-4481-918e-4cd0432ea77d" xlink:to="loc_us-gaap_RestrictedCashAndCashEquivalentsNoncurrent_45fe1207-33a0-4196-be8b-f9d78f080906" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ForeignCurrencyTransactionGainLossUnrealized_1a89abd1-e7fe-4f70-ae9c-f44a48b13f77" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ForeignCurrencyTransactionGainLossUnrealized"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RestrictedCashAndCashEquivalentsNoncurrent_45fe1207-33a0-4196-be8b-f9d78f080906" xlink:to="loc_us-gaap_ForeignCurrencyTransactionGainLossUnrealized_1a89abd1-e7fe-4f70-ae9c-f44a48b13f77" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.centrusenergy.com/role/ScheduleofIntangibleAssetsDetails" xlink:type="simple" xlink:href="leu-20240630.xsd#ScheduleofIntangibleAssetsDetails"/>
  <link:definitionLink xlink:role="http://www.centrusenergy.com/role/ScheduleofIntangibleAssetsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_cecea1d9-7198-4b6f-9b22-d8bf4dad5798" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FiniteLivedIntangibleAssetsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable_250d4ae7-78f6-4be1-a3c2-925dd0f19505" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_cecea1d9-7198-4b6f-9b22-d8bf4dad5798" xlink:to="loc_us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable_250d4ae7-78f6-4be1-a3c2-925dd0f19505" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_a165c54a-4e6a-4753-9339-848ae53e6316" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable_250d4ae7-78f6-4be1-a3c2-925dd0f19505" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_a165c54a-4e6a-4753-9339-848ae53e6316" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_a165c54a-4e6a-4753-9339-848ae53e6316_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_a165c54a-4e6a-4753-9339-848ae53e6316" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_a165c54a-4e6a-4753-9339-848ae53e6316_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_44cc2685-7034-40e4-a248-752c18b2bff4" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_a165c54a-4e6a-4753-9339-848ae53e6316" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_44cc2685-7034-40e4-a248-752c18b2bff4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ContractBasedIntangibleAssetsMember_bdcbc539-4dde-451a-8da5-45582ec26b22" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ContractBasedIntangibleAssetsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_44cc2685-7034-40e4-a248-752c18b2bff4" xlink:to="loc_us-gaap_ContractBasedIntangibleAssetsMember_bdcbc539-4dde-451a-8da5-45582ec26b22" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CustomerRelatedIntangibleAssetsMember_54224bf5-9d23-479b-a2d0-95be75b6989e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CustomerRelatedIntangibleAssetsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_44cc2685-7034-40e4-a248-752c18b2bff4" xlink:to="loc_us-gaap_CustomerRelatedIntangibleAssetsMember_54224bf5-9d23-479b-a2d0-95be75b6989e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IntangibleAssetsGrossExcludingGoodwill_b338b5d3-4a24-417c-a338-5be0aec617c5" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IntangibleAssetsGrossExcludingGoodwill"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_cecea1d9-7198-4b6f-9b22-d8bf4dad5798" xlink:to="loc_us-gaap_IntangibleAssetsGrossExcludingGoodwill_b338b5d3-4a24-417c-a338-5be0aec617c5" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization_79c59a52-634b-4f9c-846e-be609de0d596" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_cecea1d9-7198-4b6f-9b22-d8bf4dad5798" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization_79c59a52-634b-4f9c-846e-be609de0d596" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IntangibleAssetsNetExcludingGoodwill_1b67897f-281c-4c99-8992-6a35d7de7efe" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IntangibleAssetsNetExcludingGoodwill"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_cecea1d9-7198-4b6f-9b22-d8bf4dad5798" xlink:to="loc_us-gaap_IntangibleAssetsNetExcludingGoodwill_1b67897f-281c-4c99-8992-6a35d7de7efe" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.centrusenergy.com/role/ScheduleofDebtDetails" xlink:type="simple" xlink:href="leu-20240630.xsd#ScheduleofDebtDetails"/>
  <link:definitionLink xlink:role="http://www.centrusenergy.com/role/ScheduleofDebtDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentLineItems_b079e9fb-0839-440b-b269-0cfe4443b78d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentTable_355ab277-642a-4ac9-9158-8876782e5cfa" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DebtInstrumentLineItems_b079e9fb-0839-440b-b269-0cfe4443b78d" xlink:to="loc_us-gaap_DebtInstrumentTable_355ab277-642a-4ac9-9158-8876782e5cfa" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeAxis_9078551b-54dc-49bc-9f3e-a4eb8afbd71a" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LongtermDebtTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_355ab277-642a-4ac9-9158-8876782e5cfa" xlink:to="loc_us-gaap_LongtermDebtTypeAxis_9078551b-54dc-49bc-9f3e-a4eb8afbd71a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_9078551b-54dc-49bc-9f3e-a4eb8afbd71a_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_9078551b-54dc-49bc-9f3e-a4eb8afbd71a" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_9078551b-54dc-49bc-9f3e-a4eb8afbd71a_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_92b18d5d-aad4-461c-b0d7-9a5781d0d213" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_9078551b-54dc-49bc-9f3e-a4eb8afbd71a" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_92b18d5d-aad4-461c-b0d7-9a5781d0d213" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SeniorNotesMember_e28285d6-42ef-4709-9e59-fca5db789c32" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SeniorNotesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_92b18d5d-aad4-461c-b0d7-9a5781d0d213" xlink:to="loc_us-gaap_SeniorNotesMember_e28285d6-42ef-4709-9e59-fca5db789c32" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentFaceAmount_c5497633-8147-45cb-b9de-4b66739243de" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentFaceAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_b079e9fb-0839-440b-b269-0cfe4443b78d" xlink:to="loc_us-gaap_DebtInstrumentFaceAmount_c5497633-8147-45cb-b9de-4b66739243de" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherLongTermDebt_035320dd-dd3a-4a3f-b219-aafdac755fc0" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OtherLongTermDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_b079e9fb-0839-440b-b269-0cfe4443b78d" xlink:to="loc_us-gaap_OtherLongTermDebt_035320dd-dd3a-4a3f-b219-aafdac755fc0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtCurrent_14f1eb86-49df-4625-9343-ab5c7cfe86f8" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LongTermDebtCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_b079e9fb-0839-440b-b269-0cfe4443b78d" xlink:to="loc_us-gaap_LongTermDebtCurrent_14f1eb86-49df-4625-9343-ab5c7cfe86f8" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebt_18632586-a4f5-4ed6-964a-8f5c47329dae" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LongTermDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_b079e9fb-0839-440b-b269-0cfe4443b78d" xlink:to="loc_us-gaap_LongTermDebt_18632586-a4f5-4ed6-964a-8f5c47329dae" xlink:type="arc" order="3"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.centrusenergy.com/role/DebtNarrativeDetails" xlink:type="simple" xlink:href="leu-20240630.xsd#DebtNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.centrusenergy.com/role/DebtNarrativeDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_65910f19-6daa-4270-8ad2-855f3bbae8eb" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentTable_86482503-cada-4c57-981c-3b3552805528" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_65910f19-6daa-4270-8ad2-855f3bbae8eb" xlink:to="loc_us-gaap_DebtInstrumentTable_86482503-cada-4c57-981c-3b3552805528" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeAxis_d4295555-298c-48a0-bc96-a4dcfe350ce2" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LongtermDebtTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_86482503-cada-4c57-981c-3b3552805528" xlink:to="loc_us-gaap_LongtermDebtTypeAxis_d4295555-298c-48a0-bc96-a4dcfe350ce2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_d4295555-298c-48a0-bc96-a4dcfe350ce2_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_d4295555-298c-48a0-bc96-a4dcfe350ce2" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_d4295555-298c-48a0-bc96-a4dcfe350ce2_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_03e22a60-4e99-492c-9433-4e6c888cfd00" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_d4295555-298c-48a0-bc96-a4dcfe350ce2" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_03e22a60-4e99-492c-9433-4e6c888cfd00" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SeniorNotesMember_a38fb7b9-ea9e-4473-b378-e3b2a5994682" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SeniorNotesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_03e22a60-4e99-492c-9433-4e6c888cfd00" xlink:to="loc_us-gaap_SeniorNotesMember_a38fb7b9-ea9e-4473-b378-e3b2a5994682" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentInterestRateStatedPercentage_8ba37393-7d4f-475c-a836-9753b000f2b7" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentInterestRateStatedPercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_65910f19-6daa-4270-8ad2-855f3bbae8eb" xlink:to="loc_us-gaap_DebtInstrumentInterestRateStatedPercentage_8ba37393-7d4f-475c-a836-9753b000f2b7" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtCurrent_ccb8949d-59e4-4251-ae1c-bf816f29d378" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LongTermDebtCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_65910f19-6daa-4270-8ad2-855f3bbae8eb" xlink:to="loc_us-gaap_LongTermDebtCurrent_ccb8949d-59e4-4251-ae1c-bf816f29d378" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.centrusenergy.com/role/FairValueMeasurementsFinancialInstrumentsRecordedatFairValueDetails" xlink:type="simple" xlink:href="leu-20240630.xsd#FairValueMeasurementsFinancialInstrumentsRecordedatFairValueDetails"/>
  <link:definitionLink xlink:role="http://www.centrusenergy.com/role/FairValueMeasurementsFinancialInstrumentsRecordedatFairValueDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_ff5017ab-dc94-4ee3-9c6a-73993e5e2656" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_7c8396c6-9b09-41d3-ac76-c0303da0027b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_ff5017ab-dc94-4ee3-9c6a-73993e5e2656" xlink:to="loc_us-gaap_StatementTable_7c8396c6-9b09-41d3-ac76-c0303da0027b" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_267160ac-9426-4f85-b9af-94889294961d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FairValueByFairValueHierarchyLevelAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_7c8396c6-9b09-41d3-ac76-c0303da0027b" xlink:to="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_267160ac-9426-4f85-b9af-94889294961d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_267160ac-9426-4f85-b9af-94889294961d_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_267160ac-9426-4f85-b9af-94889294961d" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_267160ac-9426-4f85-b9af-94889294961d_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_0d9e2996-c23a-4279-89c4-7d3f78ef4c45" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_267160ac-9426-4f85-b9af-94889294961d" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_0d9e2996-c23a-4279-89c4-7d3f78ef4c45" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel1Member_fc777b96-0cd2-4f44-81c8-c77c09759cde" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FairValueInputsLevel1Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_0d9e2996-c23a-4279-89c4-7d3f78ef4c45" xlink:to="loc_us-gaap_FairValueInputsLevel1Member_fc777b96-0cd2-4f44-81c8-c77c09759cde" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel2Member_9d15f411-5b00-48d7-b56e-6cd9a6c33274" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FairValueInputsLevel2Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_0d9e2996-c23a-4279-89c4-7d3f78ef4c45" xlink:to="loc_us-gaap_FairValueInputsLevel2Member_9d15f411-5b00-48d7-b56e-6cd9a6c33274" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel3Member_70dfb975-809c-4aa3-a9b0-820c0b22aa0a" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FairValueInputsLevel3Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_0d9e2996-c23a-4279-89c4-7d3f78ef4c45" xlink:to="loc_us-gaap_FairValueInputsLevel3Member_70dfb975-809c-4aa3-a9b0-820c0b22aa0a" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashAndCashEquivalentsAtCarryingValue_decaec7d-688f-4e85-850e-e0a0f50d40c0" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CashAndCashEquivalentsAtCarryingValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_ff5017ab-dc94-4ee3-9c6a-73993e5e2656" xlink:to="loc_us-gaap_CashAndCashEquivalentsAtCarryingValue_decaec7d-688f-4e85-850e-e0a0f50d40c0" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashSurrenderValueFairValueDisclosure_8e0697cd-3c46-487a-990b-f45513118206" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CashSurrenderValueFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_ff5017ab-dc94-4ee3-9c6a-73993e5e2656" xlink:to="loc_us-gaap_CashSurrenderValueFairValueDisclosure_8e0697cd-3c46-487a-990b-f45513118206" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueLiabilitiesMeasuredOnRecurringBasisObligations_b178e9ea-a7d7-4456-a279-86b9d12e5019" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FairValueLiabilitiesMeasuredOnRecurringBasisObligations"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_ff5017ab-dc94-4ee3-9c6a-73993e5e2656" xlink:to="loc_us-gaap_FairValueLiabilitiesMeasuredOnRecurringBasisObligations_b178e9ea-a7d7-4456-a279-86b9d12e5019" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.centrusenergy.com/role/FairValueMeasurementsNarrativeDetails" xlink:type="simple" xlink:href="leu-20240630.xsd#FairValueMeasurementsNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.centrusenergy.com/role/FairValueMeasurementsNarrativeDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_788bf0c7-aace-4245-8cd7-db6d4fff78d1" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_92da2fe2-4297-4864-a31a-540bdc1ba55e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_788bf0c7-aace-4245-8cd7-db6d4fff78d1" xlink:to="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_92da2fe2-4297-4864-a31a-540bdc1ba55e" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeAxis_6f82ade0-279a-4508-a51f-5e8186fa44a5" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LongtermDebtTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_92da2fe2-4297-4864-a31a-540bdc1ba55e" xlink:to="loc_us-gaap_LongtermDebtTypeAxis_6f82ade0-279a-4508-a51f-5e8186fa44a5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_6f82ade0-279a-4508-a51f-5e8186fa44a5_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_6f82ade0-279a-4508-a51f-5e8186fa44a5" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_6f82ade0-279a-4508-a51f-5e8186fa44a5_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_f1879382-e886-40b6-8aa2-e5c6078aed12" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_6f82ade0-279a-4508-a51f-5e8186fa44a5" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_f1879382-e886-40b6-8aa2-e5c6078aed12" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SeniorNotesMember_9f725cf2-c115-4422-9d5e-5f4bc070652d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SeniorNotesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_f1879382-e886-40b6-8aa2-e5c6078aed12" xlink:to="loc_us-gaap_SeniorNotesMember_9f725cf2-c115-4422-9d5e-5f4bc070652d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtLongtermAndShorttermCombinedAmount_62ffb20f-8eac-4fd8-b7e2-05ae9d3afeb0" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtLongtermAndShorttermCombinedAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_788bf0c7-aace-4245-8cd7-db6d4fff78d1" xlink:to="loc_us-gaap_DebtLongtermAndShorttermCombinedAmount_62ffb20f-8eac-4fd8-b7e2-05ae9d3afeb0" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtFairValue_0381c262-aab2-4abb-916b-6911befe1ae2" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LongTermDebtFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_788bf0c7-aace-4245-8cd7-db6d4fff78d1" xlink:to="loc_us-gaap_LongTermDebtFairValue_0381c262-aab2-4abb-916b-6911befe1ae2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentFaceAmount_f86ad7e1-dc17-483a-9070-468729ef4ba6" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentFaceAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_788bf0c7-aace-4245-8cd7-db6d4fff78d1" xlink:to="loc_us-gaap_DebtInstrumentFaceAmount_f86ad7e1-dc17-483a-9070-468729ef4ba6" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentInterestRateStatedPercentage_b7b8254f-f31a-4fdf-926d-6178839aaeb1" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentInterestRateStatedPercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_788bf0c7-aace-4245-8cd7-db6d4fff78d1" xlink:to="loc_us-gaap_DebtInstrumentInterestRateStatedPercentage_b7b8254f-f31a-4fdf-926d-6178839aaeb1" xlink:type="arc" order="3"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.centrusenergy.com/role/PensionandPostretirementHealthandLifeBenefitsNarrativeDetails" xlink:type="simple" xlink:href="leu-20240630.xsd#PensionandPostretirementHealthandLifeBenefitsNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.centrusenergy.com/role/PensionandPostretirementHealthandLifeBenefitsNarrativeDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_bfae1d5a-2ddb-4156-bdbb-19885a67979d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable_3344ace4-191a-4cea-94c7-18eb19127a0d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_bfae1d5a-2ddb-4156-bdbb-19885a67979d" xlink:to="loc_us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable_3344ace4-191a-4cea-94c7-18eb19127a0d" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetirementPlanTypeAxis_5ca640e1-8aca-48d0-9721-5065fb706186" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RetirementPlanTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable_3344ace4-191a-4cea-94c7-18eb19127a0d" xlink:to="loc_us-gaap_RetirementPlanTypeAxis_5ca640e1-8aca-48d0-9721-5065fb706186" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetirementPlanTypeDomain_5ca640e1-8aca-48d0-9721-5065fb706186_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RetirementPlanTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_RetirementPlanTypeAxis_5ca640e1-8aca-48d0-9721-5065fb706186" xlink:to="loc_us-gaap_RetirementPlanTypeDomain_5ca640e1-8aca-48d0-9721-5065fb706186_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetirementPlanTypeDomain_3c36cbbe-b819-4e68-aec0-3c3eec5ea239" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RetirementPlanTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_RetirementPlanTypeAxis_5ca640e1-8aca-48d0-9721-5065fb706186" xlink:to="loc_us-gaap_RetirementPlanTypeDomain_3c36cbbe-b819-4e68-aec0-3c3eec5ea239" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PensionPlansDefinedBenefitMember_c9e4aae2-b953-4a46-b2b3-aef9f0ce2602" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PensionPlansDefinedBenefitMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RetirementPlanTypeDomain_3c36cbbe-b819-4e68-aec0-3c3eec5ea239" xlink:to="loc_us-gaap_PensionPlansDefinedBenefitMember_c9e4aae2-b953-4a46-b2b3-aef9f0ce2602" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherPostretirementBenefitPlansDefinedBenefitMember_26665be5-40df-4c75-9490-b6638b026af2" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OtherPostretirementBenefitPlansDefinedBenefitMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RetirementPlanTypeDomain_3c36cbbe-b819-4e68-aec0-3c3eec5ea239" xlink:to="loc_us-gaap_OtherPostretirementBenefitPlansDefinedBenefitMember_26665be5-40df-4c75-9490-b6638b026af2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventTypeAxis_b186c309-e856-4c0a-bd34-4b39279a2942" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SubsequentEventTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable_3344ace4-191a-4cea-94c7-18eb19127a0d" xlink:to="loc_us-gaap_SubsequentEventTypeAxis_b186c309-e856-4c0a-bd34-4b39279a2942" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventTypeDomain_b186c309-e856-4c0a-bd34-4b39279a2942_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SubsequentEventTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_SubsequentEventTypeAxis_b186c309-e856-4c0a-bd34-4b39279a2942" xlink:to="loc_us-gaap_SubsequentEventTypeDomain_b186c309-e856-4c0a-bd34-4b39279a2942_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventTypeDomain_6009b50e-6194-4173-b6a7-88491fca7a4a" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SubsequentEventTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_SubsequentEventTypeAxis_b186c309-e856-4c0a-bd34-4b39279a2942" xlink:to="loc_us-gaap_SubsequentEventTypeDomain_6009b50e-6194-4173-b6a7-88491fca7a4a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventMember_835eecbf-27d8-45ec-a490-76bd6da1e777" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SubsequentEventMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsequentEventTypeDomain_6009b50e-6194-4173-b6a7-88491fca7a4a" xlink:to="loc_us-gaap_SubsequentEventMember_835eecbf-27d8-45ec-a490-76bd6da1e777" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanBenefitObligation_a0b5c457-8de0-4ccf-8961-bb6e79daf9fc" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DefinedBenefitPlanBenefitObligation"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_bfae1d5a-2ddb-4156-bdbb-19885a67979d" xlink:to="loc_us-gaap_DefinedBenefitPlanBenefitObligation_a0b5c457-8de0-4ccf-8961-bb6e79daf9fc" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanRecognizedNetGainLossDueToSettlementsAndCurtailments1_8bdb5cc1-e85b-4bce-b6ca-8aa825eb22e7" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DefinedBenefitPlanRecognizedNetGainLossDueToSettlementsAndCurtailments1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_bfae1d5a-2ddb-4156-bdbb-19885a67979d" xlink:to="loc_us-gaap_DefinedBenefitPlanRecognizedNetGainLossDueToSettlementsAndCurtailments1_8bdb5cc1-e85b-4bce-b6ca-8aa825eb22e7" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_PortionOfDefinedBenefitPlanBenefitObligationRelatedToRemeasurement_f6213c7f-506a-4782-a3da-be043742e3f3" xlink:href="leu-20240630.xsd#leu_PortionOfDefinedBenefitPlanBenefitObligationRelatedToRemeasurement"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_bfae1d5a-2ddb-4156-bdbb-19885a67979d" xlink:to="loc_leu_PortionOfDefinedBenefitPlanBenefitObligationRelatedToRemeasurement_f6213c7f-506a-4782-a3da-be043742e3f3" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_DefinedBenefitPlanPensionPlanObligationTransferred_59dea8be-1c68-4577-924e-cf3e3c88dc13" xlink:href="leu-20240630.xsd#leu_DefinedBenefitPlanPensionPlanObligationTransferred"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_bfae1d5a-2ddb-4156-bdbb-19885a67979d" xlink:to="loc_leu_DefinedBenefitPlanPensionPlanObligationTransferred_59dea8be-1c68-4577-924e-cf3e3c88dc13" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LitigationReserve_2b7aa966-106a-4066-b759-fca3f1f8f836" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LitigationReserve"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_bfae1d5a-2ddb-4156-bdbb-19885a67979d" xlink:to="loc_us-gaap_LitigationReserve_2b7aa966-106a-4066-b759-fca3f1f8f836" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanFairValueOfPlanAssets_9904f684-511c-4893-9d8e-533f9e2a226d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DefinedBenefitPlanFairValueOfPlanAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_bfae1d5a-2ddb-4156-bdbb-19885a67979d" xlink:to="loc_us-gaap_DefinedBenefitPlanFairValueOfPlanAssets_9904f684-511c-4893-9d8e-533f9e2a226d" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_DefinedBenefitPlanPlanAssetsTransferred_644a6430-a427-4103-afa4-c6b529065f15" xlink:href="leu-20240630.xsd#leu_DefinedBenefitPlanPlanAssetsTransferred"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_bfae1d5a-2ddb-4156-bdbb-19885a67979d" xlink:to="loc_leu_DefinedBenefitPlanPlanAssetsTransferred_644a6430-a427-4103-afa4-c6b529065f15" xlink:type="arc" order="6"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.centrusenergy.com/role/PensionandPostretirementHealthandLifeBenefitsScheduleofNetBenefitCostsDetails" xlink:type="simple" xlink:href="leu-20240630.xsd#PensionandPostretirementHealthandLifeBenefitsScheduleofNetBenefitCostsDetails"/>
  <link:definitionLink xlink:role="http://www.centrusenergy.com/role/PensionandPostretirementHealthandLifeBenefitsScheduleofNetBenefitCostsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_9aec970f-5e78-4967-a367-523155ff0fe6" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable_36a7a65d-023c-4e60-b934-f3b6bb81aaac" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_9aec970f-5e78-4967-a367-523155ff0fe6" xlink:to="loc_us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable_36a7a65d-023c-4e60-b934-f3b6bb81aaac" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetirementPlanTypeAxis_8bf7cb50-30d4-4e94-83c9-8deec0eb443c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RetirementPlanTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable_36a7a65d-023c-4e60-b934-f3b6bb81aaac" xlink:to="loc_us-gaap_RetirementPlanTypeAxis_8bf7cb50-30d4-4e94-83c9-8deec0eb443c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetirementPlanTypeDomain_8bf7cb50-30d4-4e94-83c9-8deec0eb443c_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RetirementPlanTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_RetirementPlanTypeAxis_8bf7cb50-30d4-4e94-83c9-8deec0eb443c" xlink:to="loc_us-gaap_RetirementPlanTypeDomain_8bf7cb50-30d4-4e94-83c9-8deec0eb443c_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetirementPlanTypeDomain_37e819ea-8b6c-421e-8558-a08c17c7dec3" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RetirementPlanTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_RetirementPlanTypeAxis_8bf7cb50-30d4-4e94-83c9-8deec0eb443c" xlink:to="loc_us-gaap_RetirementPlanTypeDomain_37e819ea-8b6c-421e-8558-a08c17c7dec3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PensionPlansDefinedBenefitMember_0eb25428-ad0b-4d42-8b85-79e6195fe84f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PensionPlansDefinedBenefitMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RetirementPlanTypeDomain_37e819ea-8b6c-421e-8558-a08c17c7dec3" xlink:to="loc_us-gaap_PensionPlansDefinedBenefitMember_0eb25428-ad0b-4d42-8b85-79e6195fe84f" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherPostretirementBenefitPlansDefinedBenefitMember_f57879c3-9ae9-4f3e-aad5-69943a4cc96c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OtherPostretirementBenefitPlansDefinedBenefitMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RetirementPlanTypeDomain_37e819ea-8b6c-421e-8558-a08c17c7dec3" xlink:to="loc_us-gaap_OtherPostretirementBenefitPlansDefinedBenefitMember_f57879c3-9ae9-4f3e-aad5-69943a4cc96c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanServiceCost_aecec71f-d97d-4607-bd84-7a1221b53e2c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DefinedBenefitPlanServiceCost"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_9aec970f-5e78-4967-a367-523155ff0fe6" xlink:to="loc_us-gaap_DefinedBenefitPlanServiceCost_aecec71f-d97d-4607-bd84-7a1221b53e2c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanInterestCost_034f8424-6cff-432f-96b1-61e9fd600a44" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DefinedBenefitPlanInterestCost"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_9aec970f-5e78-4967-a367-523155ff0fe6" xlink:to="loc_us-gaap_DefinedBenefitPlanInterestCost_034f8424-6cff-432f-96b1-61e9fd600a44" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanAmortizationOfPriorServiceCostCredit_8f7dc7e1-5bf0-4c09-8cb2-be61d056b9de" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DefinedBenefitPlanAmortizationOfPriorServiceCostCredit"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_9aec970f-5e78-4967-a367-523155ff0fe6" xlink:to="loc_us-gaap_DefinedBenefitPlanAmortizationOfPriorServiceCostCredit_8f7dc7e1-5bf0-4c09-8cb2-be61d056b9de" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanRecognizedNetGainLossDueToSettlementsAndCurtailments1_96eab136-851f-4a08-8ea7-95e97c766867" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DefinedBenefitPlanRecognizedNetGainLossDueToSettlementsAndCurtailments1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_9aec970f-5e78-4967-a367-523155ff0fe6" xlink:to="loc_us-gaap_DefinedBenefitPlanRecognizedNetGainLossDueToSettlementsAndCurtailments1_96eab136-851f-4a08-8ea7-95e97c766867" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanExpectedReturnOnPlanAssets_3a8fd330-79c6-486c-9bd7-66cbaec3ddbb" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DefinedBenefitPlanExpectedReturnOnPlanAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_9aec970f-5e78-4967-a367-523155ff0fe6" xlink:to="loc_us-gaap_DefinedBenefitPlanExpectedReturnOnPlanAssets_3a8fd330-79c6-486c-9bd7-66cbaec3ddbb" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanNetPeriodicBenefitCost_4345c729-c78a-4a43-baf3-68fefa3aeb7d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DefinedBenefitPlanNetPeriodicBenefitCost"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_9aec970f-5e78-4967-a367-523155ff0fe6" xlink:to="loc_us-gaap_DefinedBenefitPlanNetPeriodicBenefitCost_4345c729-c78a-4a43-baf3-68fefa3aeb7d" xlink:type="arc" order="5"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.centrusenergy.com/role/StockholdersEquityNarrativeDetails" xlink:type="simple" xlink:href="leu-20240630.xsd#StockholdersEquityNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.centrusenergy.com/role/StockholdersEquityNarrativeDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockLineItems_d1cef70a-0951-4881-8e3f-953fbb1a01df" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ClassOfStockLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfStockByClassTable_da8bd186-68c0-446f-a19c-c2c4a5512d03" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ScheduleOfStockByClassTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ClassOfStockLineItems_d1cef70a-0951-4881-8e3f-953fbb1a01df" xlink:to="loc_us-gaap_ScheduleOfStockByClassTable_da8bd186-68c0-446f-a19c-c2c4a5512d03" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementEquityComponentsAxis_8a00cbf0-1c23-44d7-8027-902b72e3e013" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementEquityComponentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfStockByClassTable_da8bd186-68c0-446f-a19c-c2c4a5512d03" xlink:to="loc_us-gaap_StatementEquityComponentsAxis_8a00cbf0-1c23-44d7-8027-902b72e3e013" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_8a00cbf0-1c23-44d7-8027-902b72e3e013_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_8a00cbf0-1c23-44d7-8027-902b72e3e013" xlink:to="loc_us-gaap_EquityComponentDomain_8a00cbf0-1c23-44d7-8027-902b72e3e013_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_f570dc08-c12c-4c1d-92be-073177c359f5" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_8a00cbf0-1c23-44d7-8027-902b72e3e013" xlink:to="loc_us-gaap_EquityComponentDomain_f570dc08-c12c-4c1d-92be-073177c359f5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockMember_b3266b94-10ff-4e84-b2d7-bdf527b11b43" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PreferredStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_f570dc08-c12c-4c1d-92be-073177c359f5" xlink:to="loc_us-gaap_PreferredStockMember_b3266b94-10ff-4e84-b2d7-bdf527b11b43" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockMember_5510b960-f26d-4c7c-8aa6-6179c0ca1254" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommonStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_f570dc08-c12c-4c1d-92be-073177c359f5" xlink:to="loc_us-gaap_CommonStockMember_5510b960-f26d-4c7c-8aa6-6179c0ca1254" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdditionalPaidInCapitalMember_f06ee5e7-3bbc-4105-a3fa-af0a5ff393e5" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AdditionalPaidInCapitalMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_f570dc08-c12c-4c1d-92be-073177c359f5" xlink:to="loc_us-gaap_AdditionalPaidInCapitalMember_f06ee5e7-3bbc-4105-a3fa-af0a5ff393e5" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementClassOfStockAxis_4663df17-0ec9-4bf9-abc5-1d9343e7852f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementClassOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfStockByClassTable_da8bd186-68c0-446f-a19c-c2c4a5512d03" xlink:to="loc_us-gaap_StatementClassOfStockAxis_4663df17-0ec9-4bf9-abc5-1d9343e7852f" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_4663df17-0ec9-4bf9-abc5-1d9343e7852f_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementClassOfStockAxis_4663df17-0ec9-4bf9-abc5-1d9343e7852f" xlink:to="loc_us-gaap_ClassOfStockDomain_4663df17-0ec9-4bf9-abc5-1d9343e7852f_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_fe95ac0d-2546-4285-9727-c477c9bf8f29" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementClassOfStockAxis_4663df17-0ec9-4bf9-abc5-1d9343e7852f" xlink:to="loc_us-gaap_ClassOfStockDomain_fe95ac0d-2546-4285-9727-c477c9bf8f29" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonClassAMember_63c9e33e-0e50-48ed-b684-b6a4ed65ebb9" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommonClassAMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_fe95ac0d-2546-4285-9727-c477c9bf8f29" xlink:to="loc_us-gaap_CommonClassAMember_63c9e33e-0e50-48ed-b684-b6a4ed65ebb9" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_2b9a82ac-4310-4a5a-ad2f-1d2657b586bb" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfStockByClassTable_da8bd186-68c0-446f-a19c-c2c4a5512d03" xlink:to="loc_srt_RangeAxis_2b9a82ac-4310-4a5a-ad2f-1d2657b586bb" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_2b9a82ac-4310-4a5a-ad2f-1d2657b586bb_default" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_2b9a82ac-4310-4a5a-ad2f-1d2657b586bb" xlink:to="loc_srt_RangeMember_2b9a82ac-4310-4a5a-ad2f-1d2657b586bb_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_8f9c69c0-5a02-4ecc-a9ae-8f0524f546ac" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_2b9a82ac-4310-4a5a-ad2f-1d2657b586bb" xlink:to="loc_srt_RangeMember_8f9c69c0-5a02-4ecc-a9ae-8f0524f546ac" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_5916ae23-cfd3-4532-a332-2e0382c00284" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_8f9c69c0-5a02-4ecc-a9ae-8f0524f546ac" xlink:to="loc_srt_MinimumMember_5916ae23-cfd3-4532-a332-2e0382c00284" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_323a15e9-454e-4703-9594-53cf2aeaf4a5" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_8f9c69c0-5a02-4ecc-a9ae-8f0524f546ac" xlink:to="loc_srt_MaximumMember_323a15e9-454e-4703-9594-53cf2aeaf4a5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_ExchangeOfPreferredStockForCommonStockAndCommonStockWarrant_64afd6b7-1eb4-4495-9489-7f4574f3ee7e" xlink:href="leu-20240630.xsd#leu_ExchangeOfPreferredStockForCommonStockAndCommonStockWarrant"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_d1cef70a-0951-4881-8e3f-953fbb1a01df" xlink:to="loc_leu_ExchangeOfPreferredStockForCommonStockAndCommonStockWarrant_64afd6b7-1eb4-4495-9489-7f4574f3ee7e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_ReclassificationOfStockBasedCompensationLiabilityToEquity_153b9a1b-1c0a-4e71-bbe7-a6492a022311" xlink:href="leu-20240630.xsd#leu_ReclassificationOfStockBasedCompensationLiabilityToEquity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_d1cef70a-0951-4881-8e3f-953fbb1a01df" xlink:to="loc_leu_ReclassificationOfStockBasedCompensationLiabilityToEquity_153b9a1b-1c0a-4e71-bbe7-a6492a022311" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdjustmentsRelatedToTaxWithholdingForShareBasedCompensation_611f385e-e4c7-48c5-a3c6-989ab96106b6" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AdjustmentsRelatedToTaxWithholdingForShareBasedCompensation"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_d1cef70a-0951-4881-8e3f-953fbb1a01df" xlink:to="loc_us-gaap_AdjustmentsRelatedToTaxWithholdingForShareBasedCompensation_611f385e-e4c7-48c5-a3c6-989ab96106b6" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_AwardsUnderExecutiveIncentivePlanPeriod_b713aa17-b55b-4d31-8c6e-7eda89e0c9e4" xlink:href="leu-20240630.xsd#leu_AwardsUnderExecutiveIncentivePlanPeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_d1cef70a-0951-4881-8e3f-953fbb1a01df" xlink:to="loc_leu_AwardsUnderExecutiveIncentivePlanPeriod_b713aa17-b55b-4d31-8c6e-7eda89e0c9e4" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesNewIssues_8b00b45c-4b38-4e60-b79f-6895d97a9a92" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StockIssuedDuringPeriodSharesNewIssues"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_d1cef70a-0951-4881-8e3f-953fbb1a01df" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesNewIssues_8b00b45c-4b38-4e60-b79f-6895d97a9a92" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_StockIssuedDuringPeriodValueGross_c95ed7bc-8835-4ff1-8c60-fe3fc15e31ee" xlink:href="leu-20240630.xsd#leu_StockIssuedDuringPeriodValueGross"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_d1cef70a-0951-4881-8e3f-953fbb1a01df" xlink:to="loc_leu_StockIssuedDuringPeriodValueGross_c95ed7bc-8835-4ff1-8c60-fe3fc15e31ee" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_StockIssuedDuringPeriodValueNet_8f0ef9b0-dccf-47f6-aba1-430ea92649c3" xlink:href="leu-20240630.xsd#leu_StockIssuedDuringPeriodValueNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_d1cef70a-0951-4881-8e3f-953fbb1a01df" xlink:to="loc_leu_StockIssuedDuringPeriodValueNet_8f0ef9b0-dccf-47f6-aba1-430ea92649c3" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_DirectCostsOfStockIssuance_4fe4cf08-1dce-4fdc-a17c-aa5c5f2234a6" xlink:href="leu-20240630.xsd#leu_DirectCostsOfStockIssuance"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_d1cef70a-0951-4881-8e3f-953fbb1a01df" xlink:to="loc_leu_DirectCostsOfStockIssuance_4fe4cf08-1dce-4fdc-a17c-aa5c5f2234a6" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockParOrStatedValuePerShare_5778a6cb-9075-41ff-bdb6-770bd41fd6fc" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PreferredStockParOrStatedValuePerShare"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_d1cef70a-0951-4881-8e3f-953fbb1a01df" xlink:to="loc_us-gaap_PreferredStockParOrStatedValuePerShare_5778a6cb-9075-41ff-bdb6-770bd41fd6fc" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_PurchasePriceIncreasePerShare_3e0ca5f3-8a5a-434f-94e9-19f25d9c5156" xlink:href="leu-20240630.xsd#leu_PurchasePriceIncreasePerShare"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_d1cef70a-0951-4881-8e3f-953fbb1a01df" xlink:to="loc_leu_PurchasePriceIncreasePerShare_3e0ca5f3-8a5a-434f-94e9-19f25d9c5156" xlink:type="arc" order="9"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.centrusenergy.com/role/SegmentInformationSegmentReportingInformationDetails" xlink:type="simple" xlink:href="leu-20240630.xsd#SegmentInformationSegmentReportingInformationDetails"/>
  <link:definitionLink xlink:role="http://www.centrusenergy.com/role/SegmentInformationSegmentReportingInformationDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentReportingInformationLineItems_fc877dec-e262-4b89-b546-e09016f464ac" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SegmentReportingInformationLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_659e7fe5-4cb4-40ba-9e68-218d22e72a71" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_fc877dec-e262-4b89-b546-e09016f464ac" xlink:to="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_659e7fe5-4cb4-40ba-9e68-218d22e72a71" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductOrServiceAxis_a5ea8a28-4b93-40ac-95af-ab756ab98788" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_ProductOrServiceAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_659e7fe5-4cb4-40ba-9e68-218d22e72a71" xlink:to="loc_srt_ProductOrServiceAxis_a5ea8a28-4b93-40ac-95af-ab756ab98788" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_a5ea8a28-4b93-40ac-95af-ab756ab98788_default" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_ProductOrServiceAxis_a5ea8a28-4b93-40ac-95af-ab756ab98788" xlink:to="loc_srt_ProductsAndServicesDomain_a5ea8a28-4b93-40ac-95af-ab756ab98788_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_ebba32e3-62ea-40e1-b069-7215b7c9f65c" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_ProductOrServiceAxis_a5ea8a28-4b93-40ac-95af-ab756ab98788" xlink:to="loc_srt_ProductsAndServicesDomain_ebba32e3-62ea-40e1-b069-7215b7c9f65c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProductMember_de67ec6a-9e76-4669-9fb9-862f7b0d9e7e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ProductMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_ebba32e3-62ea-40e1-b069-7215b7c9f65c" xlink:to="loc_us-gaap_ProductMember_de67ec6a-9e76-4669-9fb9-862f7b0d9e7e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_SeparativeWorkUnitsMember_2820200b-324f-41e5-8c51-a6a781a39bf2" xlink:href="leu-20240630.xsd#leu_SeparativeWorkUnitsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ProductMember_de67ec6a-9e76-4669-9fb9-862f7b0d9e7e" xlink:to="loc_leu_SeparativeWorkUnitsMember_2820200b-324f-41e5-8c51-a6a781a39bf2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_UraniumMember_1e05eaf0-c596-4831-9d50-58df0b0bda74" xlink:href="leu-20240630.xsd#leu_UraniumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ProductMember_de67ec6a-9e76-4669-9fb9-862f7b0d9e7e" xlink:to="loc_leu_UraniumMember_1e05eaf0-c596-4831-9d50-58df0b0bda74" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ServiceMember_872c21b4-baea-4db5-a4ba-f4007735c927" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ServiceMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_ebba32e3-62ea-40e1-b069-7215b7c9f65c" xlink:to="loc_us-gaap_ServiceMember_872c21b4-baea-4db5-a4ba-f4007735c927" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementBusinessSegmentsAxis_3276f524-f32f-4881-93e8-8a1590e5706c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementBusinessSegmentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_659e7fe5-4cb4-40ba-9e68-218d22e72a71" xlink:to="loc_us-gaap_StatementBusinessSegmentsAxis_3276f524-f32f-4881-93e8-8a1590e5706c" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentDomain_3276f524-f32f-4881-93e8-8a1590e5706c_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementBusinessSegmentsAxis_3276f524-f32f-4881-93e8-8a1590e5706c" xlink:to="loc_us-gaap_SegmentDomain_3276f524-f32f-4881-93e8-8a1590e5706c_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentDomain_9403bd0d-044d-476a-8c3e-cc189a5c1641" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementBusinessSegmentsAxis_3276f524-f32f-4881-93e8-8a1590e5706c" xlink:to="loc_us-gaap_SegmentDomain_9403bd0d-044d-476a-8c3e-cc189a5c1641" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_LowEnrichedUraniumSegmentMember_8911d507-cc15-4e8a-94f7-d86ccc70843d" xlink:href="leu-20240630.xsd#leu_LowEnrichedUraniumSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_9403bd0d-044d-476a-8c3e-cc189a5c1641" xlink:to="loc_leu_LowEnrichedUraniumSegmentMember_8911d507-cc15-4e8a-94f7-d86ccc70843d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_ContractServicesSegmentMember_b36023b2-001e-4357-9b4a-bf7e8b5eb08a" xlink:href="leu-20240630.xsd#leu_ContractServicesSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_9403bd0d-044d-476a-8c3e-cc189a5c1641" xlink:to="loc_leu_ContractServicesSegmentMember_b36023b2-001e-4357-9b4a-bf7e8b5eb08a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_b1112965-fef9-4471-b7fe-232ee8c26798" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_fc877dec-e262-4b89-b546-e09016f464ac" xlink:to="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_b1112965-fef9-4471-b7fe-232ee8c26798" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GrossProfit_86866fbf-dd4e-487e-9b4a-de3b61707178" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_GrossProfit"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_fc877dec-e262-4b89-b546-e09016f464ac" xlink:to="loc_us-gaap_GrossProfit_86866fbf-dd4e-487e-9b4a-de3b61707178" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.centrusenergy.com/role/SegmentInformationScheduleOfEntityWideRevenueByMajorCustomersDetails" xlink:type="simple" xlink:href="leu-20240630.xsd#SegmentInformationScheduleOfEntityWideRevenueByMajorCustomersDetails"/>
  <link:definitionLink xlink:role="http://www.centrusenergy.com/role/SegmentInformationScheduleOfEntityWideRevenueByMajorCustomersDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EntityWideRevenueMajorCustomerLineItems_df2f9a10-82c2-4bfc-b23c-70d301903ff1" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EntityWideRevenueMajorCustomerLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfEntityWideRevenueByMajorCustomersByReportingSegmentsTable_1aebba94-c292-4ef1-81b7-2576c46c2c8b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ScheduleOfEntityWideRevenueByMajorCustomersByReportingSegmentsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_EntityWideRevenueMajorCustomerLineItems_df2f9a10-82c2-4bfc-b23c-70d301903ff1" xlink:to="loc_us-gaap_ScheduleOfEntityWideRevenueByMajorCustomersByReportingSegmentsTable_1aebba94-c292-4ef1-81b7-2576c46c2c8b" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MajorCustomersAxis_c2dec5d6-e47e-47e7-bf44-650898dbee32" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_MajorCustomersAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfEntityWideRevenueByMajorCustomersByReportingSegmentsTable_1aebba94-c292-4ef1-81b7-2576c46c2c8b" xlink:to="loc_srt_MajorCustomersAxis_c2dec5d6-e47e-47e7-bf44-650898dbee32" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_NameOfMajorCustomerDomain_c2dec5d6-e47e-47e7-bf44-650898dbee32_default" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_NameOfMajorCustomerDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_MajorCustomersAxis_c2dec5d6-e47e-47e7-bf44-650898dbee32" xlink:to="loc_srt_NameOfMajorCustomerDomain_c2dec5d6-e47e-47e7-bf44-650898dbee32_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_NameOfMajorCustomerDomain_67d3f529-ca50-4445-9c44-3b7e20f19471" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_NameOfMajorCustomerDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_MajorCustomersAxis_c2dec5d6-e47e-47e7-bf44-650898dbee32" xlink:to="loc_srt_NameOfMajorCustomerDomain_67d3f529-ca50-4445-9c44-3b7e20f19471" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_CustomerAMember_e3296344-3402-4ef0-a127-aa0781a8b66b" xlink:href="leu-20240630.xsd#leu_CustomerAMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_NameOfMajorCustomerDomain_67d3f529-ca50-4445-9c44-3b7e20f19471" xlink:to="loc_leu_CustomerAMember_e3296344-3402-4ef0-a127-aa0781a8b66b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_CustomerBMember_9f5a186f-082b-408a-a7e6-d8948083a41b" xlink:href="leu-20240630.xsd#leu_CustomerBMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_NameOfMajorCustomerDomain_67d3f529-ca50-4445-9c44-3b7e20f19471" xlink:to="loc_leu_CustomerBMember_9f5a186f-082b-408a-a7e6-d8948083a41b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_CustomerCMember_ecee5327-243e-41d9-a67b-d321f1ee49a5" xlink:href="leu-20240630.xsd#leu_CustomerCMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_NameOfMajorCustomerDomain_67d3f529-ca50-4445-9c44-3b7e20f19471" xlink:to="loc_leu_CustomerCMember_ecee5327-243e-41d9-a67b-d321f1ee49a5" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_CustomerEMember_50ffacfd-c336-4f77-969c-1ccd4a7619ad" xlink:href="leu-20240630.xsd#leu_CustomerEMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_NameOfMajorCustomerDomain_67d3f529-ca50-4445-9c44-3b7e20f19471" xlink:to="loc_leu_CustomerEMember_50ffacfd-c336-4f77-969c-1ccd4a7619ad" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_CustomerDMember_7b93ed6f-0954-4d12-b8f7-050fbc0380af" xlink:href="leu-20240630.xsd#leu_CustomerDMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_NameOfMajorCustomerDomain_67d3f529-ca50-4445-9c44-3b7e20f19471" xlink:to="loc_leu_CustomerDMember_7b93ed6f-0954-4d12-b8f7-050fbc0380af" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementBusinessSegmentsAxis_a2b07d10-ce30-4187-aa66-4ea025e73c09" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementBusinessSegmentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfEntityWideRevenueByMajorCustomersByReportingSegmentsTable_1aebba94-c292-4ef1-81b7-2576c46c2c8b" xlink:to="loc_us-gaap_StatementBusinessSegmentsAxis_a2b07d10-ce30-4187-aa66-4ea025e73c09" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentDomain_a2b07d10-ce30-4187-aa66-4ea025e73c09_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementBusinessSegmentsAxis_a2b07d10-ce30-4187-aa66-4ea025e73c09" xlink:to="loc_us-gaap_SegmentDomain_a2b07d10-ce30-4187-aa66-4ea025e73c09_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentDomain_5e51473d-53ca-4fef-ba4d-ab8070441728" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementBusinessSegmentsAxis_a2b07d10-ce30-4187-aa66-4ea025e73c09" xlink:to="loc_us-gaap_SegmentDomain_5e51473d-53ca-4fef-ba4d-ab8070441728" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_LowEnrichedUraniumSegmentMember_f2fb16dd-2631-4d81-826b-0b121298ef52" xlink:href="leu-20240630.xsd#leu_LowEnrichedUraniumSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_5e51473d-53ca-4fef-ba4d-ab8070441728" xlink:to="loc_leu_LowEnrichedUraniumSegmentMember_f2fb16dd-2631-4d81-826b-0b121298ef52" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_leu_ContractServicesSegmentMember_9ec81757-336f-49bf-a11a-9cb1215042e9" xlink:href="leu-20240630.xsd#leu_ContractServicesSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_5e51473d-53ca-4fef-ba4d-ab8070441728" xlink:to="loc_leu_ContractServicesSegmentMember_9ec81757-336f-49bf-a11a-9cb1215042e9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Revenues_64e8af39-0a95-41a6-95b4-7ccaceac570f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_Revenues"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EntityWideRevenueMajorCustomerLineItems_df2f9a10-82c2-4bfc-b23c-70d301903ff1" xlink:to="loc_us-gaap_Revenues_64e8af39-0a95-41a6-95b4-7ccaceac570f" xlink:type="arc" order="0"/>
  </link:definitionLink>
</link:linkbase>
